Response of methanogenic archaea to Late Pleistocene and Holocene climate changes in the Siberian Arctic
Response of methanogenic archaea to Late Pleistocene and Holocene climate changes in the Siberian Arctic
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DOI：
10.1029/2011gb004238
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发表时间：
2013-04-01
影响因子：
5.2
通讯作者：
Wagner, Dirk
中科院分区：
文献类型：
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作者：
Bischoff, Juliane;Mangelsdorf, Kai;Wagner, Dirk
为了探讨过去多年冻土沉积物中甲烷动态与气候变化的关系，我们研究了西伯利亚北极地区晚更新世和全新世沉积物中产甲烷群落的丰度、组成和甲烷产量。我们在西伯利亚东北部勒拿河三角洲库伦纳赫岛42ka多年冻土序列中发现了晚更新世和全新世沉积物中甲烷浓度增加的间隔。通过对产甲烷古菌的16S核糖体核糖核酸基因分析发现，古菌生命标记物（完整的磷脂醚）数量的增加和遗传指纹的多样性表明，目前生活的和可能活跃的产甲烷古菌主要存在于全新世沉积物的不同层中，但也存在于17米深的冻土中。通过培养实验证实了潜在的产甲烷活性。通过比较甲烷浓度、微生物培养实验、产甲烷菌的基因分析和微生物生命标记（完整的磷脂酯和醚）与已经部分降解的膜脂，如古醇和类异戊二烯甘油二烷基甘油四醚，我们证明了古醇可能代表了过去产甲烷的古菌的信号。
In order to investigate the link between the methane dynamics in permafrost deposits and climate changes in the past, we studied the abundance, composition, and methane production of methanogenic communities in Late Pleistocene and Holocene sediments of the Siberian Arctic. We detected intervals of increased methane concentrations in Late Pleistocene and Holocene deposits along a 42ka old permafrost sequence from Kurungnakh Island in the Lena Delta (northeast Siberia). Increased amounts of archaeal life markers (intact phospholipid ethers) and a high variety in genetic fingerprints detected by 16S ribosomal ribonucleic acid gene analyses of methanogenic archaea suggest presently living and presumably active methanogenic archaea in distinct layers predominantly in Holocene deposits, but also in deep frozen ground at 17m depth. Potential methanogenic activity was confirmed by incubation experiments. By comparing methane concentrations, microbial incubation experiments, gene analysis of methanogens, and microbial life markers (intact phospholipid esters and ethers) to already partly degraded membrane lipids, such as archaeol and isoprenoid glycerol dialkyl glycerol tetraethers, we demonstrated that archaeol likely represents a signal of past methanogenic archaea.