Aquaporin-3 gene and protein expression in sun-protected human skin decreases with skin ageing
Aquaporin-3 gene and protein expression in sun-protected human skin decreases with skin ageing
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受阳光照射的人体皮肤中水通道蛋白 3 基因和蛋白质的表达随着皮肤老化而降低
DOI：
10.1111/j.1440-0960.2010.00629.x
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发表时间：
2010-05-01
影响因子：
2
通讯作者：
Xie, Hongfu
中科院分区：
文献类型：
--
作者：
Li, Ji;Tang, Hua;Xie, Hongfu
背景/目标：水通道蛋白 3 (AQP3) 是一种与皮肤水合有关的蛋白质。 AQP3缺失小鼠皮肤相对干燥，皮肤弹性降低，去除角质层后屏障功能恢复较慢，老年人皮肤中也存在这种现象。皮肤老化的一个特征是皮肤的含水量和屏障功能的变化。我们研究了水通道蛋白3（AQP3）在不同年龄组非日晒人体皮肤、正常人角质形成细胞和成纤维细胞中的表达，以进一步了解AQP3与皮肤内在衰老的关系。方法：采用免疫组化、免疫细胞化学、逆转录聚合酶链式反应检测水通道蛋白3（AQP3）在不同年龄的正常人皮肤、正常人表皮角质形成细胞（NHEK）和皮肤成纤维细胞中的表达。 (RT-PCR) 和蛋白质印迹。样本来自 60 名不同年龄的患者：< 20 岁、30-45 岁和 > 60 岁。每个年龄组均采集 20 份皮肤活检，其中 6 份用于角质形成细胞/成纤维细胞培养。结果：皮肤和 NHEK 样本中的 AQP3 均随着年龄的增加而下降。我们证明 3 个年龄组之间 AQP3 表达存在显着差异 (P < 0.05)。在成纤维细胞中，> 60岁组的AQP3表达水平显着低于30-45岁组（P < 0.05）和< 20岁组（P < 0.05），两个年轻组之间无显着性差异（P > 0.05）。结论：AQP3可能参与非日晒人体皮肤的内在衰老过程。
Backgroud/Objectives:Aquaporin 3 (AQP3) is a protein implicated in skin hydration. AQP3 null mice have relatively dry skin, reduced skin elasticity, and delayed recovery of barrier function after removal of the stratum corneum which is also present in skin of old people. A feature of skin aging is the change in both water content and barrier function of the skin. We investigated the expression of aquaporin 3 in non sun-exposed human skin, normal human keratinocytes and fibroblasts from different age groups to further understand the relationship between AQP3 and intrinsic skin aging.Methods:We investigated the expression of aquaporin3 (AQP3) in normal human skin, normal human epidermal keratinocytes (NHEK) and skin fibroblast of different ages by immunohistochemistry, immunocytochemistry, the reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. The samples were derived from 60 patients of varying ages: < 20 years of age, 30-45 years and > 60 years of age. Twenty skin biopsies, 6 for keratinocyte/fibroblast cultures, were taken from each age group.Results:AQP3 decreased with increasing age in both skin and NHEK samples. We demonstrated significant differences in AQP3 expression between the 3 age groups (P < 0.05). In fibroblasts, AQP3 expression levels were significantly lower in the > 60 year olds compared to 30-45 year olds (P < 0.05) and < 20 year olds (P < 0.05), there was no significant difference between the two younger groups (P > 0.05).Conlusions:AQP3 may be involved in the intrinsic aging process of non sun-exposed human skin.