TbMP57 is a 3′ terminal uridylyl transferase (TUTase) of the Trypanosoma brucei editosome
TbMP57 is a 3′ terminal uridylyl transferase (TUTase) of the Trypanosoma brucei editosome
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DOI：
10.1016/s1097-2765(03)00185-0
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发表时间：
2003-06-01
期刊：
影响因子：
16
通讯作者：
Stuart, K
中科院分区：
文献类型：
--
作者：
Ernst, NL;Panicucci, B;Stuart, K
RNA编辑通过尿苷(U)插入和缺失产生成熟的锥虫线粒体mRNAs。在插入编辑中，我们通过3‘末端尿苷酰转移酶(TUTase)活性被添加到前mRNA中。我们报告了一种TUTase活性的鉴定，它与体外编辑一起纯化，并由完整的编辑体蛋白TbMP57催化。TbMP57主要催化将单个U加到单链(Ss)RNA上，并将由引导RNA指定的数目的我们添加到插入编辑样底物上。TbMP57不同于以前发现的TUTase，它将许多我们添加到单链RNA中，我们发现它既不是一个稳定的编辑体成分，也不会将我们添加到类似编辑的底物中。重组的TbMP57与编辑体蛋白TbMP81特异地相互作用，这种作用增强了TUTase的活性。这些结果表明，TbMP57在编辑过程中催化U加成到前mRNA。
RNA editing produces mature trypanosome mitochondrial mRNAs by uridylate (U) insertion and deletion. In insertion editing, Us, are added to the pre-mRNA by a 3' terminal uridylyl transferase (TUTase) activity. We report the identification of a TUTase activity that co-purifies with in vitro editing and is catalyzed by the integral editosome protein TbMP57. TbMP57 catalyzes the addition of primarily a single U to single-stranded (ss) RNA and adds the number of Us specified by a guide RNA to insertion editing-like substrates. TbMP57 is distinct from a previously identified TUTase that adds many Us to ssRNA and which we find is neither a stable editosome component nor does it add Us to editing-like substrates. Recombinant TbMP57 specifically interacts with the editosome protein TbMP81, and this interaction enhances the TUTase activity. These results suggest that TbMP57 catalyzes U addition to pre-mRNA during editing.