Two novel transcripts expressed in pea dormant axillary buds.
Two novel transcripts expressed in pea dormant axillary buds.
复制标题
在豌豆休眠腋芽中表达的两个新转录本。
DOI：
10.1093/pcp/41.3.274
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发表时间：
2000
影响因子：
4.9
通讯作者：
H. Mori
中科院分区：
文献类型：
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作者：
Y. Madoka;H. Mori
为了阐明顶端优势的分子机制，对豌豆休眠腋芽中优势表达基因的表达模式进行了研究。CV.阿拉斯加）幼苗进行了调查。利用差异显示技术从休眠腋芽cDNA文库中分离到两个cDNA克隆，分别命名为cPsAD 1和cPsAD 2，其对应的基因分别命名为PsAD 1和PsAD 2。PsAD 1的氨基酸序列由87个氨基酸残基组成，氨基末端富含甘氨酸残基。蛋白质数据库的搜索未能找到任何类似于PsAD 1蛋白质的序列，除了富含甘氨酸的区域。北方印迹分析表明，PsAD 1 mRNA主要在休眠的腋芽中积累，顶芽去顶后其表达量迅速下降。原位杂交分析表明，PsAD 1 mRNA定位于顶端分生组织，原形成层，和叶原基在休眠的腋芽，有能力生长出来，但其生长暂时中止。也就是说，PsAD 1基因的表达与腋芽的休眠密切相关。PsAD 2的推导的氨基酸序列包含98个氨基酸残基，并且与先前表征的蛋白质不相似。PsAD 2 mRNA在休眠的腋芽、根、成熟的小叶和伸长的茎中积累，表明PsAD 2不仅参与了腋芽的休眠，还参与了各种组织的非生长状态。
To elucidate the molecular mechanism of apical dominance, the expression patterns of genes that are preferentially expressed in dormant axillary buds of pea (Pisum sativum L. cv. Alaska) seedlings were investigated. We isolated two cDNA clones, cPsAD1 and cPsAD2 whose corresponding genes were named PsAD1 and PsAD2, from a cDNA library of dormant axillary buds using the differential display method. The deduced amino acid sequence of PsAD1 contains 87 residues and is rich in glycine residues in the amino terminal region. A search of the protein databases failed to find any sequences similar to PsAD1 protein except for the glycine-rich region. Northern blot analyses showed that PsAD1 mRNA mainly accumulated in dormant axillary buds and that its amount rapidly decreased after decapitation of the terminal bud. In situ hybridization analyses indicated that PsAD1 mRNA was localized in the apical meristem, procambia, and leaf primordia in dormant axillary buds that were competent to grow out but whose growth was temporarily suspended. That is, the expression of the PsAD1 gene is closely associated with the dormancy of axillary buds. The deduced amino acid sequence of PsAD2 contains 98 amino acid residues and is not similar to those of previously characterized proteins. PsAD2 mRNA accumulated in dormant axillary buds, roots, mature leaflets and elongated stems, suggesting that PsAD2 is involved in not only the dormancy of axillary buds but also the non-growing state in various tissues.
Madoka，Y.，Mori，H.：“顶端优势释放后豌豆腋芽中 PsAD1 蛋白的顶峰消失”植物细胞生理学。
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