PRE- AND POSTSYNAPTIC LOCALIZATION OF NMDA RECEPTOR SUBUNITS AT HIPPOCAMPAL MOSSY FIBRE SYNAPSES
PRE- AND POSTSYNAPTIC LOCALIZATION OF NMDA RECEPTOR SUBUNITS AT HIPPOCAMPAL MOSSY FIBRE SYNAPSES
复制标题
DOI：
10.1016/j.neuroscience.2012.10.061
复制
发表时间：
2013-01-29
期刊：
影响因子：
3.3
通讯作者：
Gundersen, V.
中科院分区：
文献类型：
--
作者：
Berg, L. K.;Larsson, M.;Gundersen, V.
N-甲基-D-天冬氨酸 (NMDA) 型谷氨酸受体参与海马苔藓纤维 -CA3 锥体神经元突触的突触可塑性。 NMDA 受体亚基在这种突触类型上的超微结构定位尚不清楚。通过包埋后电子显微镜免疫金细胞化学，我们发现 NMDA 受体亚基 GluN1、GluN2A、GluN2B、GluN2C 和 GluN2D 位于苔藓纤维的突触后膜以及 CA3 复发关联连合突触中。在苔藓纤维中，GluN1、GluN2B 和 GluN2D 标记模式表明这些亚基也位于神经末梢膜和苔藓纤维轴突的突触前。与经常性关联连合突触相比，GluN3B 主要存在于苔藓纤维突触中，显示出突触前标记模式。总之，虽然 GluN1、GluN2A、GluN2B 和 GluN2D 的突触后定位与 CA3 苔藓纤维突触 NMDA 受体依赖性长期增强 (LTP) 的最新发现非常一致，但我们提出突触前 GluN1、GluN2B、GluN2D 和 GluN3B 亚基可能参与可塑现象，例如某些类型的 LTP 和苔藓纤维反复生长。 (C) 2012 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
The N-methyl-D-aspartate (NMDA) type of glutamate receptors is involved in synaptic plasticity in hippocampal mossy fibre-CA3 pyramidal neuron synapses. The ultrastructural localization of NMDA receptor subunits at this synapse type is not known. By postembedding electron microscopic immunogold cytochemistry we show that the NMDA receptor subunits GluN1, GluN2A, GluN2B, GluN2C and GluN2D are located in postsynaptic membranes of mossy fibre as well as CA3 recurrent associational commissural synapses. In the mossy fibres the GluN1, GluN2B and GluN2D labelling patterns suggested that these subunits were located also presynaptically in nerve terminal membranes and in mossy fibre axons. GluN3B was predominantly present in mossy fibre synapses as compared to recurrent associational commissural synapses, showing a presynaptic labelling pattern. In conclusion, while the postsynaptic localization of GluN1, GluN2A, GluN2B, and GluN2D is in good agreement with the recent finding of NMDA receptor-dependent long term potentiation (LTP) at CA3 mossy fibre synapses, we propose that presynaptic GluN1, GluN2B, GluN2D and GluN3B subunits could be involved in plastic phenomena such as certain types of LTP and recurrent mossy fibre growth. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.