Expression, purification, and characterization of the recombinant pepsin inhibitor from Ascaris suum.
Expression, purification, and characterization of the recombinant pepsin inhibitor from Ascaris suum.
复制标题
猪蛔虫重组胃蛋白酶抑制剂的表达、纯化和表征。
DOI：
10.1007/978-1-4615-5373-1_54
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发表时间：
1998
影响因子：
--
通讯作者：
Dunn,BM
中科院分区：
文献类型：
--
作者：
Zalatoris,J;Rao-Naik,C;Fecho,G;Girdwood,K;Kay,J;Dunn,BM
迄今为止，只有少数天然的天冬氨酸蛋白酶抑制剂被发现。已经显示来自细菌来源的少量肽抑制该蛋白酶家族的成员。1 - 2在马铃薯中发现了天冬氨酸蛋白酶的较大蛋白质抑制剂3（组织蛋白酶D抑制剂），在酵母中，4和肠道线虫蛔虫。5 - 7在其他寄生线虫中也发现了类似的基因。8蛔虫天冬氨酸蛋白酶抑制剂（PI-3）是一种16.7 kDa的蛋白质，特异性抑制胃蛋白酶、胃蛋白酶和组织蛋白酶E，但抑制程度较低。该抑制剂具有六个半胱氨酸残基，形成三个二硫键，并且含有螺旋和链结构基序。
Only a few natural inhibitors of aspartic proteinases have been discovered to date. A small number of peptides from bacterial origins have been shown to inhibit members of this protease family.1-2Larger proteinaceous inhibitors of aspartic proteases have been discovered in the potato3(cathepsin D inhibitor), in yeast,4and the intestinal nematodeAscaris.5–7Similar genes have been discovered in other parasitic nematodes.8TheAscarisaspartic proteinase inhibitor (PI-3) is a 16.7 kDa protein that specifically inhibits pepsin, gastricsin, and, less strongly, cathepsin E. This inhibitor possesses six cysteine residues which form three disulfide bridges, and it contains helical and strand structural motifs.