Brain extracellular matrix affects AMPA receptor lateral mobility and short-term synaptic plasticity
Brain extracellular matrix affects AMPA receptor lateral mobility and short-term synaptic plasticity
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DOI：
10.1038/nn.2338
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发表时间：
2009-07-01
影响因子：
25
通讯作者：
Gundelfinger, Eckart D.
中科院分区：
文献类型：
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作者：
Frischknecht, Renato;Heine, Martin;Gundelfinger, Eckart D.
成熟CNS中的许多突触被致密的细胞外基质（ECM）包裹。使用单粒子跟踪和荧光恢复后，光漂白，我们发现，这种网状ECM形成大鼠原代神经元，作为AMPA型谷氨酸受体的横向扩散屏障的表面隔间。酶清除ECM增加突触外受体扩散和突触AMPA受体的交换。全细胞膜片钳记录显示，作为ECM去除的功能结果，配对脉冲比率增加。这些结果表明，由ECM形成的表面隔室阻碍AMPA受体的侧向扩散，因此可能调节短期突触可塑性。
Many synapses in the mature CNS are wrapped by a dense extracellular matrix (ECM). Using single-particle tracking and fluorescence recovery after photobleaching, we found that this net-like ECM formed surface compartments on rat primary neurons that acted as lateral diffusion barriers for AMPA-type glutamate receptors. Enzymatic removal of the ECM increased extrasynaptic receptor diffusion and the exchange of synaptic AMPA receptors. Whole-cell patch-clamp recording revealed an increased pairedpulse ratio as a functional consequence of ECM removal. These results suggest that the surface compartments formed by the ECM hinder lateral diffusion of AMPA receptors and may therefore modulate short-term synaptic plasticity.