A Highly Sensitive, Dual-Readout Assay Based on Gold Nanoparticles for Organophosphorus and Carbamate Pesticides
A Highly Sensitive, Dual-Readout Assay Based on Gold Nanoparticles for Organophosphorus and Carbamate Pesticides
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基于金纳米粒子的高灵敏度双读数测定法，用于有机磷和氨基甲酸酯农药
DOI：
10.1021/ac300545p
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发表时间：
2012-05-01
影响因子：
7.4
通讯作者：
Jiang, Xingyu
中科院分区：
文献类型：
--
作者：
Liu, Dingbin;Chen, Wenwen;Jiang, Xingyu
本报告提出了一种高灵敏度，罗丹明B覆盖的金纳米粒子（RB-AuNP）为基础的测定与双读出（比色和荧光）检测有机磷和氨基甲酸酯农药在复杂的解决方案。检测机制是基于这样的事实，即这些农药可以抑制乙酰胆碱酯酶（AChE）的活性，从而防止硫代胆碱的产生（它使RB-AuNP溶液变蓝，同时使RB的荧光不猝灭）。RB-AuNP溶液的颜色保持红色，RB的荧光保持淬灭。通过使用这种双读出分析，几种农药包括西维因、二嗪农、马拉硫磷和甲拌磷的最低检测浓度分别为0.1、0.1、0.3和1 μ g/L，所有这些都远低于欧盟农药数据库以及美国农业部（USDA）报告的最大残留限量（MRL）.该分析允许检测农产品和河水等真实的样品中的农药。将该方法用于食品中农药残留的检测，结果与高效液相色谱法（HPLC）结果一致。因此，这种简单的检测方法适用于检测复杂样品中的农药，特别是与其他便携式平台相结合。
This report presents a highly sensitive, rhodamine B-covered gold nanoparticle (RB-AuNP) -based assay with dual readouts (colorimetric and fluorometric) for detecting organophosphorus and carbamate pesticides in complex solutions. The detection mechanism is based on the fact that these pesticides can inhibit the activity of acetylcholinesterase (AChE), thus preventing the generation of thiocholine (which turns the RB-AuNP solutions blue and unquenches the fluorescence of RB simultaneously). The color of the RB-AuNP solution remains red and the fluorescence of RB remains quenched. By use of this dual-readout assay, the lowest detectable concentrations for several kinds of pesticides including carbaryl, diazinon, malathion, and phorate were measured to be 0.1, 0.1, 0.3, and 1 mu g/L, respectively, all of which are much lower than the maximum residue limits (MRL) as reported in the European Union pesticides database as well as those from the U.S. Department Agriculture (USDA). This assay allows detection of pesticides in real samples such as agricultural products and river water. The results in detecting pesticide residues collected from food samples via this method agree well with those from high-performance liquid chromatography (HPLC). This simple assay is therefore suitable for sensing pesticides in complex samples, especially, in combination with other portable platforms.