Molecular analysis of an EL4 cell line that expresses H-2Db but not H-2Kb or beta 2-microglobulin.
Molecular analysis of an EL4 cell line that expresses H-2Db but not H-2Kb or beta 2-microglobulin.
复制标题
对表达 H-2Db 但不表达 H-2Kb 或 β2-微球蛋白的 EL4 细胞系进行分子分析。
DOI：
10.1073/pnas.82.9.2950
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发表时间：
1985
影响因子：
11.1
通讯作者：
Rajan,TV
中科院分区：
文献类型：
--
作者：
Potter,TA;Zeff,RA;Schmitt-Verhulst,AM;Rajan,TV
EL4/Mar is a variant cell line that expresses H-2Db but neither H-2Kb nor beta 2-microglobulin (beta 2m). Southern and RNA blot analysis and immunoprecipitation of metabolically labeled proteins established that the B2m gene(s), beta 2m mRNA, and beta 2m protein are normal in this cell line. Somatic cell hybridization showed that the defect in this cell line was in the synthesis of H-2Kb, and RNA blot analysis with an H-2Kb specific oligonucleotide established that the H-2Kb gene(s) in this cell line was not transcribed into a stable mRNA species. The apparent absence of beta 2m on the surface of this cell line suggests that there may be some feature of the H-2Db molecule that allows it to be expressed in the absence of detectable beta 2m.
