The influence of gamete co-incubation length on the in vitro fertility and sex ratio of bovine bulls with different penetration speed.
The influence of gamete co-incubation length on the in vitro fertility and sex ratio of bovine bulls with different penetration speed.
复制标题
配子共孵化时间对不同穿透速度公牛体外受精率和性比的影响
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
B. Gasparrini
中科院分区：
文献类型：
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作者：
Abdul Sattar;M. Rubessa;S. D. Francesco;V. Longobardi;R. Palo;L. Zicarelli;G. Campanile;B. Gasparrini
这项工作的目的是评估精子穿透速度是否与体外生育力相关，以及是否适应配子共孵育长度的公牛的动力学提高体外生育力和影响性比。将体外成熟的卵母细胞与来自四种不同公牛的精子共孵育（A-D）。在不同的授精后（p.i.）4、8、12、16和20 h）时，将卵母细胞样品固定并用DAPI染色用于核检查，而将剩余的卵母细胞转移到培养物中以评估胚胎发育。通过PCR对所产生的囊胚进行性别鉴定。两个公牛（A和B）具有比其他公牛（C和D）更快的动力学，如在感染后4小时记录的更高的穿透率所示。(43 A、B、C和D公牛的平均日增重分别为30%、11%和6%（p<0.01）。公牛之间的动力学差异并不反映其体外受精。多精受精的发生率在更快穿透的公牛中更高（公牛A、B、C和D分别为36%、24%、16%和4%; p<0.01），并且在更长的共孵育时间中更高（分别在感染后4、8、12、16和20小时为0%、16%、19%、30%和34%; p<0.01）。个体公牛的可交配性可以通过使共孵时间长度适应它们的穿透速度来提高。8小时的精子卵母细胞共孵育长度确保了最大的囊胚产量为两个更快的穿透公牛。相反，共孵16 h才能显著提高（p<0.01）两种慢牛的卵裂率。具有更快动力学的公牛并没有改变胚胎性别比对男性。在注射后4、8、12、16和20 h记录的雌性/雄性（F/M）比值分别为2.1、1.4、1.2、1.3和1.6。
The objectives of this work were to evaluate whether the sperm penetration speed is correlated to the in vitro fertility and whether adapting the gamete co-incubation length to the kinetics of the bull improves in vitro fertility and affects the sex ratio. In vitro matured oocytes were co-incubated with spermatozoa from four different bulls (A-D). At various post-insemination (p.i.) times (4, 8, 12, 16 and 20 h), samples of oocytes were fixed and stained with DAPI for nuclei examination, while the remaining ones were transferred into culture to evaluate embryo development. The blastocysts produced were sexed by PCR. Two bulls (A and B) had faster kinetics than the others (C and D), as shown by the higher penetration rates recorded at 4 h p.i. (43%, 30%, 11% and 6%, respectively for bulls A, B, C and D; p<0.01). The differences in the kinetics among bulls did not reflect their in vitro fertility. The incidence of polyspermy was higher for faster penetrating bulls (36%, 24%, 16% and 4%, respectively for bulls A, B, C and D; p<0.01) and at longer co-incubation times (0%, 16%, 19%, 30% and 34%, respectively at 4, 8, 12, 16 and 20 h p.i.; p<0.01). The fertilizing ability of individual bulls may be improved by adapting the co-incubation length to their penetration speed. A sperm-oocyte co-incubation length of 8 h ensured the greatest blastocyst yields for the two faster penetrating bulls. On the contrary, 16 h co-incubation was required to increase (p<0.01) cleavage rate of the two slower bulls. Bulls with a faster kinetics did not alter the embryo sex ratio towards males. The female/male (F/M) ratios recorded were 2.1, 1.4, 1.2, 1.3 and 1.6, respectively at 4, 8, 12, 16 and 20 h p.i.
影响因子：
3.6
作者：
Sutovsky, P;Navara, CS;Schatten, G
通讯作者：
Schatten, G