Increased grain yield and micronutrient concentration in transgenic winter wheat by ectopic expression of a barley sucrose transporter
Increased grain yield and micronutrient concentration in transgenic winter wheat by ectopic expression of a barley sucrose transporter
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DOI：
10.1016/j.jcs.2014.01.017
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发表时间：
2014-07-01
影响因子：
3.8
通讯作者：
Weber, Hans
中科院分区：
文献类型：
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作者：
Saalbach, Isolde;Mora-Ramirez, Isabel;Weber, Hans
优化同化物向穗部的分配是提高小麦产量潜力的重要途径。新的冬小麦品系（HOSUT），异位表达大麦大麦大麦醇溶蛋白B1启动子控制的大麦蔗糖转运蛋白HvSUT1被用来评估在田间条件下提高蔗糖吸收能力对籽粒产量和品质的潜力。三个独立的HOSUT系在微型地块中生长了三年。与未转化的对照小麦Certo相比，每小区的谷物产量显著增加了28%，同时每小区的蛋白质产量更高，铁和锌浓度更高。千粒重增加，表明HvSUT1的表达增加了单个籽粒库强度。HOSUT谷粒较大，显示增加的谷粒宽度和较小程度的谷粒长度，表明在谷粒在子代控制下生长的阶段的转基因效应。穗粒数平均下降15%，蛋白质含量平均下降5%，尤其是谷蛋白。总的来说，尽管每穗粒数和蛋白质含量有一些补偿效应，HOSUT系产生了显着的产量优势。研究结果有助于理解籽粒大小和数量的决定因素及其与籽粒库强度的关系，并可能确定小麦产量潜力的限制。(C)2014爱思唯尔有限公司版权所有。
Optimising assimilate partitioning to spikes is important to increase wheat yield potential. Novel winter wheat lines (HOSUT), ectopically expressing barley sucrose transporter HvSUT1 controlled by the barley Hordein B1 promoter were used to evaluate the potential of improved sucrose uptake capacity on grain yield and quality under field-like conditions. Three independent HOSUT lines were grown over three years in micro-plots. Grain yield per plot was significantly increased by 28%, together with higher protein yield per plot and higher iron and zinc concentration when compared to the non-transformed control wheat Certo. Thousand grain weight was enhanced, indicating that expression of HvSUT1 increases individual grain sink strength. HOSUT grains are larger, display increased grain width and to a lesser extent grain length, indicating transgene effects at a stage when grains grow under filial control. Grain number per spike was decreased by 15% and protein contents by 5%, on average, especially that of glutenins. Overall, despite some compensating effects on grain number per spike and protein contents, HOSUT lines generate a significant yield advantage. The findings can contribute to understanding determinants of grain size and number and its relationship to grain sink strength and might identify limitations of wheat yield potential. (C) 2014 Elsevier Ltd. All rights reserved.