Exocrine Organs Imaged in Aqueous Solution by Atmospheric Scanning Electron Microscopy (ASEM).
Exocrine Organs Imaged in Aqueous Solution by Atmospheric Scanning Electron Microscopy (ASEM).
复制标题
通过大气扫描电子显微镜 (ASEM) 对水溶液中的外分泌器官进行成像。
DOI：
10.1093/jmicro/dfw050
复制
发表时间：
2016
期刊：
影响因子：
1.8
通讯作者：
C.Sato.
中科院分区：
文献类型：
--
作者：
T.Yamazawa;N.Nakamura;Y.Mikami;H.Sekiya;M.Sato;C.Sato.
腮腺、下颌骨腺和舌下腺是唾液腺的三大组成部分。这些腺体通过连接腺体和口腔的主要排泄管分泌浆液、粘液或混合唾液。在Sjögren综合征中，初级外分泌腺和泪腺（LG）或SG的分泌量减少，导致眼和口干。在这里，我们报告了用大气扫描电子显微镜（ASEM）直接观察水溶液中的组织[1,2]。将SG和LG用戊二醛固定，切片成薄片，用磷钨酸（PTA）染色，倒置扫描电镜从下观察自由基清除剂d -葡萄糖溶液，光学显微镜从上引导定位组织亚结构。扫描电镜显示了2 ~ 3µm的试样厚度。在SG和LG中，分泌囊泡和其他细胞器在高分辨率下清晰成像，前者可以根据PTA染色[3]的程度进行分类。这些方法应与钙信号[4]相结合，分析排泄器官的生理功能。结果表明溶液内ASEM对组织学的有用性，高通量ASEM有望用于外分泌相关疾病的诊断，例如Sjögren综合征。
The parotid glands, submandibular glands and sublingual glands are three major salivary glands (SG). These glands secrete serous, mucous or mixed saliva via the proper main excretory ducts connecting the glandular bodies with the oral cavity. In Sjögren’s syndrome, secretory production is diminished for the primary exocrine gland and the lacrimal glands (LG) or SG, leading to dry eye and mouth. Here, we report the direct observation of tissue in aqueous solution by atmospheric scanning electron microscopy (ASEM)[1, 2]. SG and LG were glutaraldehyde fixed, sectioned into slabs, stained with phosphotungstic acid (PTA), and observed in radical scavenger D-glucose solution from below by an inverted scanning electron microscopy, guided by optical microscopy from above to target the tissue substructures. A 2 to 3-µm specimen thickness was visualized by the SEM. In SG and LG, secretory vesicles and other organelles were clearly imaged at high resolution, and the former could be classified according to the degree of PTA staining [3]. These methods should be correlated with calcium signaling [4] to analyze physiological function of the excretory organs. The results suggest the usefulness of in-solution ASEM for histology, and the high-throughput ASEM is promising for the diagnosis of exocrine-related diseases, eg, Sjögren’s syndrome.