Immobilization of Gold Nanoparticles on Living Cell Membranes upon Controlled Lipid Binding
Immobilization of Gold Nanoparticles on Living Cell Membranes upon Controlled Lipid Binding
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DOI：
10.1021/nl101454a
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发表时间：
2010-08-01
期刊：
影响因子：
10.8
通讯作者：
Feldmann, Jochen
中科院分区：
文献类型：
--
作者：
Ba, Haojin;Rodriguez-Fernandez, Jessica;Feldmann, Jochen
我们提出了一种通用的、可控的方法来将金纳米颗粒固定在活细胞的表面，同时保持了原始胶体的传感和光热能力。我们的方法是基于Au纳米颗粒在细胞孵育之前受控和选择性地结合到磷脂上。我们发现，在细胞存在的情况下，脂类结合的Au纳米粒被输送到细胞膜上，由于脂类结合，它们的扩散相当缓慢，空间上受到限制。这种方法避免了非特异性膜标记，适用于多种类型的胶体和细胞，从而为活细胞膜的受控等离子体和光热研究提供了一个平台。
We present a versatile and controlled route to immobilize gold nanoparticles (NPs) on the surface of living cells, while preserving the sensing and optothermal capabilities of the original colloid. Our approach is based on the controlled and selective binding of Au NPs to phospholipids prior to cell incubation. We show that in the presence of the cells the lipid-bound Au NPs are delivered to the cellular membrane and that their diffusion is rather slow and spatially limited, as a result of lipid binding. Avoiding nonspecific membrane labeling, this approach is of general application to several types of colloids and cells and thereby provides a platform for controlled plasmonic and optothermal investigations of living cell membranes.