Vascular endothelial growth factor localization in human ovary and fallopian tubes: possible role in reproductive function and ovarian cyst formation.
Vascular endothelial growth factor localization in human ovary and fallopian tubes: possible role in reproductive function and ovarian cyst formation.
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DOI：
10.1210/jcem.81.1.8550777
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
J. D. Gordon;S. Mesiano;C. Zaloudek;R. Jaffe
中科院分区：
文献类型：
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作者：
J. D. Gordon;S. Mesiano;C. Zaloudek;R. Jaffe
血管内皮生长因子（VEGF）是一种有效的血管生成因子，也可以增加血管通透性。我们假设VEGF在女性卵巢循环血管生成中起调节作用，其增加血管通透性的能力可能是卵巢囊肿输卵管流出物产生和液体形成的重要因素。为了验证这些假设，我们评估了绝经前（n = 10）和绝经后（n = 4）女性卵巢和输卵管中VEGF的表达。使用兔多克隆抗体对人VEGF进行免疫组化分析。在绝经前妇女的正常卵巢中，健康卵泡内的VEGF定位于鞘细胞层，在颗粒细胞层中检测到极少的VEGF肽。VEGF在闭锁卵泡或退行性黄体中未表达。然而，在所有检查的标本中，在高度血管化的黄体中观察到强烈的VEGF免疫染色。在绝经后妇女的正常卵巢中，VEGF仅在上皮包涵性囊肿和浆液性囊腺瘤中检测到。在绝经前和绝经后妇女的标本中，输卵管腔上皮以及输卵管和卵巢门内小血管和大血管内壁的平滑肌细胞和周细胞显示出VEGF特异性染色。基于这些数据，我们认为在生殖生命中，VEGF通过介导血管生成在卵巢卵泡和黄体的生长和维持中发挥作用。此外，输卵管管腔上皮内的VEGF可能增加血管通透性，调节输卵管管腔分泌物。同样，良性卵巢肿瘤上皮内的VEGF可能有助于卵巢囊肿的液体形成。
Vascular endothelial growth factor (VEGF) is a potent angiogenic factor that also increases vascular permeability. We hypothesized that VEGF plays a role in the regulation of cyclic ovarian angiogenesis in women, and that its ability to increase vascular permeability may be an important factor in the production of fallopian tube effluent and fluid formation in ovarian cysts. To examine these hypotheses, we assessed VEGF expression in ovaries and fallopian tubes from premenopausal (n = 10) and postmenopausal (n = 4) women. Immunohistochemical analysis for VEGF was performed using a rabbit polyclonal antibody directed against human VEGF. In normal ovaries from premenopausal women, VEGF within healthy follicles was localized to the thecal cell layer, with minimal VEGF peptide detected in the granulosa cell layer. VEGF was not expressed in atretic follicles or a degenerating corpus luteum. However, intense VEGF immunostaining was observed within the highly vascularized corpora lutea in all specimens examined. In normal ovaries from postmenopausal women, VEGF was detected only in epithelial inclusion cysts and a serous cystadenoma. In specimens from both pre- and postmenopausal women, the luminal epithelium of the fallopian tube as well as smooth muscle cells and pericytes lining small and large blood vessels within the tube and hilum of the ovary exhibited specific staining for VEGF. Based on these data, we suggest that during reproductive life, VEGF plays a role in the growth and maintenance of the ovarian follicle and corpus luteum by mediating angiogenesis. In addition, VEGF within the fallopian tube luminal epithelium may increase vascular permeability and modulate tubal luminal secretions. Similarly, VEGF in the epithelial lining of benign ovarian neoplasms may contribute to fluid formation in ovarian cysts.