Depletion with PC61 mAb before Toxoplasma gondii infection eliminates mainly Tregs in BALB/c mice, but activated cells in C57BL/6J mice
Depletion with PC61 mAb before Toxoplasma gondii infection eliminates mainly Tregs in BALB/c mice, but activated cells in C57BL/6J mice
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DOI：
10.1111/j.1574-695x.2011.00805.x
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发表时间：
2011-08-01
影响因子：
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通讯作者：
Saavedra, Rafael
中科院分区：
文献类型：
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作者：
Patricia Tenorio, Eda;Fernandez, Jacquelina;Saavedra, Rafael
在感染期间体内调节性T细胞（Tregs）的分析对于理解免疫反应调节至关重要。使用抗cd25单克隆抗体（mAb）消除Tregs的耗尽实验已被广泛用于此目的，尽管这种方法也可能导致活化T细胞的消除。我们在本文中表明，在弓形虫感染前用抗cd25单抗治疗可消除耐药BALB/c和易感C57BL/6J小鼠品系中不同模式的细胞亚群。在BALB/c小鼠中，注射PC61 mAb可消除大多数treg，而在C57BL/6J小鼠中，治疗会消耗其他激活的亚群[自然杀伤（NK）、B和CD4(+) T细胞]。这种差异是在后一种菌株中观察到的戏剧性细胞激活的结果，而在前一种菌株中则没有。这里报告的消耗的不同影响表明，为了避免误导性的结论，必须对每个模型进行仔细分析。
Analysis of regulatory T cells (Tregs) in vivo during infection is crucial for the understanding of immune response modulation. Depletion experiments using anti-CD25 monoclonal antibody (mAb) in order to eliminate Tregs have been widely used for this purpose despite the fact that this approach may also lead to the elimination of activated T cells. We show in this paper that treatment with anti-CD25 mAb before Toxoplasma gondii infection eliminates a different pattern of cell subsets in the resistant BALB/c and the susceptible C57BL/6J mouse strain. Injection with PC61 mAb leads to the elimination of most Tregs in BALB/c mice, while in C57BL/6J animals, treatment depletes other activated subsets [natural killer (NK), B and CD4(+) T cells]. This difference is a consequence of the dramatic cell activation observed in the latter, but not in the former strain. The different effect of the depletion reported here demonstrates that careful analysis in each model is mandatory in order to avoid misleading conclusions.