Removal of methyl parathion from artificial off-gas using a bioreactor containing a constructed microbial consortium
Removal of methyl parathion from artificial off-gas using a bioreactor containing a constructed microbial consortium
复制标题
使用含有构建的微生物群落的生物反应器去除人工废气中的甲基对硫磷
DOI：
10.1021/es702631x
复制
发表时间：
2008-03-15
影响因子：
11.4
通讯作者：
Liu, Junxin
中科院分区：
文献类型：
--
作者：
Li, Lin;Yang, Chao;Liu, Junxin
甲基异丙磷（MP）是一种高毒有机磷农药，广泛应用于农业作物保护. MP在生产和含MP废水处理过程中，会向大气中释放，对周边社区造成严重危害。组装了由工程微生物和天然对硝基苯酚（PNP）降解剂组成的财团，用于MP的完全矿化。我们对大肠杆菌BL 21（DE 3）进行基因工程改造，使其能够过表达甲基精氨酸水解酶（MPH）。此外，我们分离出了苍白杆菌属菌株LL-1，其利用MP水解产物PNP作为唯一的碳源、氮源和能源。共培养物有效地水解0.2 mM MP，并防止悬浮培养物中PNP的积累。建立了一个实验室规模的双菌群生物反应器，用于处理含MP的人工尾气。生物反应器在75天的时间内保持超过98%的平均MP去除效率，并且从MP水解产生的PNP完全降解，表明实现了MP的完全矿化。将降解菌群与生物反应器连接的策略可以提供用于处理含有MP以及其他PNP取代的有机磷酸酯的废气流的物理化学减排技术的替代方案。
Methyl parathion (MP), a highly toxic organophosphorus pesticide, was widely used for agriculture crop protection. During the production of MP and the process of MP-containing wastewater treatment, MP can release into the atmosphere and will do great harm to adjacent communities. A consortium comprised of an engineered microorganism and a natural rho-nitrophenol (PNP) degrader was assembled for complete mineralization of MP. We genetically engineered Escherichia coli BL21 (DE3) enabling the overexpression of methyl parathion hydrolase (MPH). In addition, we isolated Ochrobactrum sp. strain LL-1 that utilized PNP, a product of MP hydrolysis, as the sole carbon, nitrogen, and energy source. The coculture effectively hydrolyzed 0.2 mM MP and prevented the accumulation of PNP in suspended culture. A laboratory-scale bioreactor containing the dual-species consortium was developed for the treatment of artificial off-gas containing MP. The bioreactor maintained over 98% of average MP removal efficiency over a 75 day period, and PNP produced from hydrolysis of MP was degraded completely, indicating that complete mineralization of MP was achieved. The strategy of linking degrading consortium to a bioreactor may provide an alternative to physicochemical abatement technologies for the treatment of waste-gas streams containing MP as well as other PNP-substituted organophosphates.