Size heterogeneity of human prolactin in plasma and pituitary extracts.
Size heterogeneity of human prolactin in plasma and pituitary extracts.
复制标题
血浆和垂体提取物中人催乳素的大小异质性。
DOI：
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发表时间：
1974
影响因子：
5.8
通讯作者：
A. Frantz
中科院分区：
文献类型：
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作者：
H. Suh;A. Frantz
摘要在迄今为止检测的所有血浆样品中，经SephadexG-100凝胶过滤后，通过免疫测定法鉴定了循环人催乳素的两个离散组分。主峰，称为“小”催乳素，洗脱与纯化的131 I-催乳素一致。较少延迟的第二个峰，称为“大”催乳素，出现在人血清白蛋白和卵清蛋白之间。“大”和“小”催乳素组分在多个稀释度下测定时，在免疫学上无法区分。在正常人、嫌色细胞腺瘤和特发性乳腺炎患者的血浆样本中，“大”催乳素占总免疫反应性的8-20%。无论是TRH刺激还是左旋多巴抑制都不会引起血浆中“大”与“小”催乳素比例的重大变化，这在正常人和肿瘤患者中是相似的。在妊娠受试者的血浆中观察到最高量的“大”催乳素（范围16-31%）。这两种成分似乎也存在于垂体提取物和垂体培养液中。
ABSTRACT Two discrete components of circulating human prolactin have been identified by immunoassay after gel filtration of Sephadex G-100 in all plasma samples so far examined. The major peak, termed “little” prolactin, eluted coincident with purified 131I-prolactin. The less retarded second peak, termed “big” prolactin, emerged between human serum albumin and ovalbumin. “Big” and “little” prolactin fractions were indistinguishable immunologically when assayed at multiple dilutions. “Big” prolactin constituted 8–20% of the total immunoreactivity in plasma samples from normal subjects, patients with chromophobe adenomas, and idiopathic galactorrhea. Neither TRH stimulation nor l-dopa suppression produced major changes in the plasma ratios of “big” to “little” prolactin, which were similar in normals and tumor patients. The highest amounts of “big” prolactin (range 16–31%) were seen in plasmas from pregnant subjects. Both components also appear to be present in pituitary extracts and pituitary culture medi...