In vivo simultaneous tracing and Ca2+ imaging of local neuronal circuits
In vivo simultaneous tracing and Ca2+ imaging of local neuronal circuits
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DOI：
10.1016/j.neuron.2007.02.018
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发表时间：
2007-03-15
期刊：
影响因子：
16.2
通讯作者：
Chen, Wei R.
中科院分区：
文献类型：
--
作者：
Nagayama, Shin;Zeng, Shaoqun;Chen, Wei R.
关于大脑的一个中心问题是，嵌入在复杂局部网络中的大量神经元是如何处理信息的。解决这个问题不仅需要同时监测许多神经元的功能动力学，而且还需要在神经元回路的背景下解释这种活动模式。在这里，我们介绍了一种多功能的方法加载Ca2+，指标在体内通过局部电穿孔。利用这种方法，可以在神经元群体水平和精确的亚细胞分辨率下至树突棘和轴突终扣两者上进行Ca2+成像。这使得二尖瓣细胞气味诱发的整体活动进行分析，同时揭示其特定的连接到不同的肾小球。浦肯野细胞树突和交叉的平行纤维的Colabeling允许Ca2+，突触前终扣和突触后树突的成像。因此，这种方法提供了一个前所未有的能力，在体内可视化活性细胞系综和跟踪其潜在的局部神经元回路。
A central question about the brain is how information is processed by large populations of neurons embedded in intricate local networks. Answering this question requires not only monitoring functional dynamics of many neurons simultaneously, but also interpreting such activity patterns in the context of neuronal circuitry. Here, we introduce a versatile approach for loading Ca2+, indicators in vivo by local electroporation. With this method, Ca2+, imaging can be performed both at neuron population level and with exquisite subcellular resolution down to dendritic spines and axon boutons. This enabled mitral cell odor-evoked ensemble activity to be analyzed simultaneously with revealing their specific connectivity to different glomeruli. Colabeling of Purkinje cell dendrites and intersecting parallel fibers allowed Ca2+, imaging of both presynaptic boutons and postsynaptic dendrites. This approach thus provides an unprecedented capability for in vivo visualizing active cell ensembles and tracing their underlying local neuronal circuits.