BACTERICIDAL ACTIVITIES OF LYSOZYME AND APROTININ AGAINST GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA RELATED TO THEIR BASIC CHARACTER
BACTERICIDAL ACTIVITIES OF LYSOZYME AND APROTININ AGAINST GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA RELATED TO THEIR BASIC CHARACTER
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DOI：
10.1111/j.1365-2672.1992.tb01821.x
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发表时间：
1992-03-01
期刊：
影响因子：
--
通讯作者：
WILD, P
中科院分区：
文献类型：
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作者：
PELLEGRINI, A;THOMAS, U;WILD, P
研究了抑肽酶（一种蛋白酶抑制剂，可能也是牛类的防御分子）和鸡蛋清溶菌酶（称为胞壁酶）的杀菌特性。 在存在抑肽酶或溶菌酶的情况下孵育各种细菌表明，这两种蛋白质在不添加补体或 EDTA 的情况下都能杀死革兰氏阳性和革兰氏阴性细菌。 二硫苏糖醇使两种蛋白质变性并没有导致其杀菌效力丧失。 对与溶菌酶或抑肽酶一起孵育的大肠杆菌进行电子显微镜检查显示，细菌细胞质逐渐分解。 通过免疫金标记，在受影响的细胞质中证实了抑肽酶和溶菌酶。 结果表明，溶菌酶的杀菌效力不仅归因于胞壁酶活性，还归因于其阳离子和疏水特性。 抑肽酶的杀菌活性可能也与这两种特性有关，而不是与其作为蛋白酶抑制剂的活性有关。
Bactericidal properties of aprotinin, a proteinase inhibitor and possibly a defence molecule in bovine species, and of chicken egg white lysozyme, known as muramidase, were investigated. Incubation of various bacteria in the presence of either aprotinin or lysozyme showed that both proteins killed Gram-positive as well as Gram-negative bacteria without addition of complement or EDTA. Denaturation of the two proteins by dithiothreitol did not lead to loss of their bactericidal potency. Electron microscopic examination of Escherichia coli incubated either with lysozyme or aprotinin revealed that the bacterial cytoplasms gradually disintegrated. Both aprotinin and lysozyme were demonstrated within the affected cytoplasm by immunogold labelling. The results suggest that the bactericidal potency of lysozyme is not only due to muramidase activity but also to its cationic and hydrophobic properties. The bactericidal activity of aprotinin is probably also related to both these properties rather than to its activity as proteinase inhibitor.