A genome-wide analysis of the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) gene family in barley (Hordeum vulgare L.)
A genome-wide analysis of the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) gene family in barley (Hordeum vulgare L.)
复制标题
大麦 (Hordeum vulgare L.) 不对称叶 2/横向器官边界 (AS2/LOB) 基因家族的全基因组分析
DOI：
10.1631/jzus.b1500277
复制
发表时间：
2016-10-01
影响因子：
5.1
通讯作者：
Xu, Ru-gen
中科院分区：
文献类型：
--
作者：
Guo, Bao-jian;Wang, Jun;Xu, Ru-gen
ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) genes are a family of plant specific transcription factors, which play an important role in the regulation of plant lateral organ development and metabolism. However, a genome-wide analysis of the AS2/LOB gene family is still not available for barley. In the present study, 24 AS2-like (ASL)/LOB domain (LBD) genes were identified based on the barley (Hordeum vulgare L.) genome sequence. A phylogenetic tree of ASL/LBD proteins from barley, Arabidopsis, maize, and rice was constructed. The ASL/LBD genes were classified into two classes, class I and class II, which were divided into five and two subgroups, respectively. Genes homologous in barley and Arabidopsis were analyzed. In addition, the structure and chromosomal locations of the genes were analyzed. Expression profiles indicated that barley HvASL/LBD genes exhibit a variety of expression patterns, suggesting that they are involved in various aspects of physiological and developmental processes. This genome-wide analysis of the barley AS2/LOB gene family contributes to our understanding of the functions of the AS2/LOB gene family.中文概要目 的从大麦全基因组中鉴定AS2/LOB 基因家族,并进行基因进化、基因结构、染色体定位以及组织、表达分析,为大麦ASL/LBD 基因进一步功能研究与鉴定奠定基础。创新点首次在大麦全基因组水平上分析AS2/LOB 基因家族,并对部分基因的功能进行预测和分析。方 法利用大麦基因组数据库,通过生物信息学手段,鉴定大麦AS2/LOB 基因家族成员;采用MEGA6软件进行系统进化树分析; 利用GSDS 及MapDraw 工具进行基因结构及染色体定位分析;利用已有的大麦RNAseq 数据进行组织表达谱分析。结 论通过全基因组分析,大麦AS2/LOB 家族基因包括24 个成员,在进化上分为两大类,7 个亚家族,分布于大麦7 条染色体上,组织表达模式具有多样性,与已经报道的ASL/LBD 基因具有良好的同源性。这些信息为大麦AS2/LOB 基因家族的功能分析奠定了基础。