Measurements of the dielectric properties of peripheral blood mononuclear cells and trophoblast cells using AC electrokinetic techniques
Measurements of the dielectric properties of peripheral blood mononuclear cells and trophoblast cells using AC electrokinetic techniques
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DOI：
10.1016/s0925-4439(99)00115-5
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发表时间：
2000-03-17
影响因子：
6.2
通讯作者：
Thomas, MR
中科院分区：
文献类型：
--
作者：
Chan, KL;Morgan, H;Thomas, MR
从母体循环中分离滋养层细胞可为遗传异常的产前诊断提供有价值的诊断工具。这已经尝试使用抗体方法，但由于抗体的非特异性，母体细胞污染仍然是一个问题。我们研究了介电电泳分离法作为从混合外周血单个核细胞中分离滋养层细胞的方法的潜力。为了确定这种方法的潜力，使用介电电泳交叉和单细胞电旋转法测量了滋养层细胞和混合外周血单个核细胞的介电性质。介电电泳交叉数据和电旋转数据都给出了外周血单个核细胞的平均比膜电容为11.5 mF m（-2）。使用三种不同方法制备的滋养层细胞具有较高的平均比膜电容，范围为13-18 mF m（-2）。细胞类型之间的电容的差异可以被利用为基础的AC电动为基础的系统从外周血单核细胞的滋养层细胞的分离。(C)2000爱思唯尔科技有限公司。保留所有权利。
The separation of trophoblast cells from the maternal circulation could provide a valuable diagnostic tool for prenatal diagnosis of genetic abnormalities. This has been attempted using antibody methods, but due to non-specificity of the antibodies, maternal cell contamination remains a problem. We have investigated the potential of dielectrophoretic separation methods as a means of isolating trophoblast cells from mixed peripheral blood mononuclear cells, To determine the potential of this method the dielectric properties of trophoblast cells and mixed peripheral blood mononuclear cells were measured using dielectrophoretic crossover and single cell electrorotation methods. Both dielectrophoretic crossover data and electrorotation data gave an average specific membrane capacitance of the peripheral blood mononuclear cells of 11.5 mF m(-2). Trophoblast cells prepared using three different methods had a higher average specific membrane capacitance in the range 13-18 mF m(-2). The differences in capacitance between the cell types could be exploited as the basis of an AC electrokinetic-based system for the separation of trophoblast cells from peripheral blood mononuclear cells. (C) 2000 Elsevier Science B.V. All rights reserved.