Urinary beta-FSH subunit concentrations in perimenopausal and postmenopausal women: a biomarker for ovarian reserve.
Urinary beta-FSH subunit concentrations in perimenopausal and postmenopausal women: a biomarker for ovarian reserve.
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围绝经期和绝经后妇女尿 β-FSH 亚基浓度：卵巢储备功能的生物标志物。
DOI：
10.1097/01.gme.0000070524.74726.18
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Muse,Ken
中科院分区：
文献类型：
--
作者：
Liu,JamesH;Kao,Lily;Rebar,RobertW;Muse,Ken
目的在围绝经期(围绝经期)和绝经后(后)妇女人群中，建立一种对垂体促卵泡激素(FSH)分泌的综合评估方法，并验证这些测量方法。设计在这项横断面研究中，170名围绝经期妇女和20名围绝经期妇女收集了第一次排尿的晨尿样本，并在同一天采集了单一血样。为了进行比较，11名中年育龄妇女在一个月经周期内采集了尿样。此外，一名48.5岁妇女在绝经过渡期间连续4年采集每日尿样。测定尿样雌酮葡萄糖醛酸苷(E1G)和孕二醇-3-葡萄糖醛酸苷(PDG)，并将其归一化为肌酐。结果围产期和产后妇女的平均年龄(±SD)分别为48.1±3.0和5 2.8±4.1岁。围产期和产后妇女血清促性腺激素水平分别为9.5±5.8IU/L和79.3±32IU/L(P<；0.001)。围产期尿β-FSH/Cr平均值为1.8±1.2 ng/mg，术后为9.3±4.5 ng/mg(P<；0.001)。平均雌二醇、E_1G/Cr和PDG/Cr水平在两组间也有显著差异。围产期(r=0.584，P=0.007)和围产期(r=0.54P<；0.001)患者血清卵泡刺激素水平与尿β-FSH/Cr呈高度正相关，但两者之间的重叠较小。Pdg/Cr与尿β-FSH/Cr呈显著正相关(r=0.581，P=0.002)。尿β-FSH/Cr值与E1G/Cr值、雌二醇值无相关性。在围绝经期患者中，收集了4年的每日尿样，在卵泡期尿液β-FSH/Cr水平逐渐升高，到第4年，尿液中β-FSH/Cr水平持续升高，几乎处于高水平。结论尿β-FSH亚单位测定是监测绝经过渡期卵巢功能的有用指标。尿液中游离β-FSH亚单位浓度反映了脑下垂体促性腺激素的分泌，可作为卵巢储备的生物标志物。
ObjectiveThe purpose of this study was to develop an integrative assessment of pituitary follicle-stimulating hormone (FSH) secretion and to validate these measurements in a population of perimenopausal (PERI) and postmenopausal (POST) women.DesignIn this cross-sectional study, 170 POST and 20 PERI women collected first-void morning urine samples and had a single blood sample drawn on the same day. For comparison, 11 midreproductive-aged women had urine samples collected for one menstrual cycle. In addition, one 48.5-year-old woman collected daily urine samples for 4 consecutive years during her menopausal transition. Urine samples were assayed for estrone glucuronide (E 1 G) and pregnanediol-3-glucuronide (PdG) and were normalized to creatinine. An ELISA assay was developed for measurement of the free β-FSH subunit in urine.ResultsMean age (±SD) of the PERI and POST women were 48.1±3.0 and 52.8±4.1 years, respectively. Mean serum FSH levels were 9.5±5.8 and 79.3±32 IU/L (P< 0.001) in the PERI and POST women. Mean urinary β-FSH/Cr for the PERI were 1.8±1.2 ng/mg; for the POST, 9.3±4.5 ng/mg (P< 0.001). Mean estradiol, E 1 G/Cr, and PdG/Cr levels were also significantly different between the two groups. There was a high correlation between serum FSH and urinary β-FSH/Cr for the PERI (r= 0.584, P= 0.007) and POST (r= 0.54, P< 0.001), with minimal overlap in the urinary β-FSH/Cr levels between the PERI and POST groups. A significant correlation between PdG/Cr and urinary β-FSH/Cr was observed for POST (r= 0.581, P= 0.002). No correlation was seen between urinary β-FSH/Cr and E 1 G/Cr or estradiol levels. In the perimenopausal participant, who collected 4 years of daily urine samples, urinary β-FSH/Cr levels progressively increased during the follicular phase and, by the fourth year, there were persistent, almost tonically high elevations of β-FSH/Cr in the urine.ConclusionsUrinary β-FSH subunit measurements are a useful marker for monitoring ovarian function during the menopausal transition. Urinary free β-FSH subunit concentrations reflect pituitary FSH secretion and serve as a biomarker for ovarian reserve.