Antisera specific for cell lines with mixed neuronal and glial properties.
Antisera specific for cell lines with mixed neuronal and glial properties.
复制标题
针对具有混合神经元和神经胶质特性的细胞系的特异性抗血清。
DOI：
10.1016/s0012-1606(81)80017-6
复制
发表时间：
1981
影响因子：
2.7
通讯作者：
Stallcup,WB
中科院分区：
文献类型：
--
作者：
Wilson,SS;Baetge,EE;Stallcup,WB
抗血清是针对“假神经元”和“假神经胶质”细胞系的例子提出的，之所以这样命名是因为它们分别表现出神经元和神经胶质细胞系的一些但不是全部特征。抗血清确定了几种抗原，这些抗原为这些不寻常的细胞系与更经典的神经和神经胶质细胞系的关系提供了线索。例如，N4抗原被三个假神经元细胞系中的每一个和十个神经元细胞系中的七个表达，但没有被假神经胶质或神经胶质细胞系表达。这进一步建立了假神经元与神经元的关系，这首先通过在两种细胞类型上发现相似的Na+和K+通道来指示。然而，仅在三个假神经元系上发现的N5抗原强调了两种细胞类型是可区分的这一事实。NG1和NG2抗原仅在假神经元和假神经胶质细胞系上发现，进一步确立了这些细胞系与更直接的神经元和神经胶质细胞系之间的区别。NG1存在于一个假神经元和七个假神经胶质细胞中的两个上，而NG2存在于所有三个假神经元和四个缺乏NG1的假神经胶质细胞上。NG1和NG2的这种分布强化了我们的印象，即假神经元和假胶质细胞可能彼此相关，可能是发育谱系的一部分。由于这两种抗原都存在于正常大鼠脑中，通过定量吸收测定，因此可以使用免疫组织化学技术在细胞水平上绘制它们的位置。
Antisera were raised against examples of “pseudo-neuronal” and “pseudo-glial” cell lines—so named because they exhibited some but not all of the characteristics of neuronal and glial cell lines, respectively. The antisera defined several antigens that provide clues to the relationship of these unusual cell lines to the more classical nerve and glial lines. For example, the N4 antigen was expressed by each of the three pseudo-neuronal cell lines and by seven of the ten neuronal cell lines but by none of the pseudo-glial or glial cell lines. This further established a relationship of pseudo-neurons to neurons that was first indicated by the finding of similar Na+and K+channels on the two cell types. Nevertheless, the N5 antigen, found only on the three pseudo-neuronal lines, emphasized the fact that two cell types are distinguishable. The NG1 and NG2 antigens were found only on the pseudo-neuronal and pseudo-glial cell lines, further establishing the distinctions between these cell lines and the more straightforward neuronal and glial lines. NG1 was present on one pseudo-neuron and two of the seven pseudo-glia, while NG2 was found on all three pseudo-neurons and four of the pseudo-glia that lacked NG1. This distribution of NG1 and NG2 reinforced our impression that pseudo-neurons and pseudo-glia might be related to each other, possibly as part of a developmental lineage. Since both of these antigens are present in normal rat brain, as determined by quantitative absorption, it may be possible to map their location at the cellular level using immunohistochemical techniques.