Chronic Effects of Fluoxetine on Danio rerio: A Biochemical and Behavioral Perspective
Chronic Effects of Fluoxetine on Danio rerio: A Biochemical and Behavioral Perspective
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氟西汀对斑马鱼的慢性影响：生化和行为的角度
DOI：
10.3390/app12042256
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Miguel Oliveira
中科院分区：
文献类型：
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作者：
Daniela Correia;Inês Domingues;M. Faria;Miguel Oliveira
氟西汀是一种广泛用于治疗抑郁和焦虑状态的抗抑郁药。由于其在中枢神经系统中的作用方式(选择性5-羟色胺再摄取抑制物(SSRI))，它对非靶标生物产生毒性，导致对它们的生存有害的变化。本研究通过测定氟西汀对斑马鱼幼鱼(Danio Rerio)的生化指标(生物转化Ⅱ期谷胱甘肽S转移酶、神经递质乙酰胆碱酯酶、能量代谢乳酸脱氢酶和氧化应激谷胱甘肽过氧化物酶)和行为终点(游泳行为、社会行为和触觉)的影响，从生化角度观察了氟西汀对斑马鱼神经传递和能量代谢的影响。在行为层面上，探索性行为和社交行为没有受到影响。然而，暴露的鱼在光明和黑暗时期观察到游动模式的变化(运动活动减少)。总体而言，数据显示，长期接触氟西汀的幼鱼可能会出现行为变化，影响它们对环境应激源的反应能力以及与其他鱼类的相互作用。
Fluoxetine is an antidepressant widely used to treat depressive and anxiety states. Due to its mode of action in the central nervous system (selective serotonin reuptake inhibitor (SSRI)), it becomes toxic to non-target organisms, leading to changes that are harmful to their survival. In this work, the effects of fluoxetine on juvenile zebrafish (Danio rerio) were evaluated, assessing biochemical (phase II biotransformation—glutathione S-transferase (GST), neurotransmission—acetylcholinesterase (ChE), energy metabolism—lactate dehydrogenase (LDH), and oxidative stress—glutathione peroxidase (GPx)) and behavior endpoints (swimming behavior, social behavior, and thigmotaxis) after 21 days exposure to 0 (control), 0.1, 1 and 10 µg/L. Biochemically, although chronic exposure did not induce significant effects on neurotransmission and energy metabolism, GPx activity was decreased after exposure to 10 µg/L of fluoxetine. At a behavioral level, exploratory and social behavior was not affected. However, changes in the swimming pattern of exposed fish were observed in light and dark periods (decreased locomotor activity). Overall, the data show that juvenile fish chronically exposed to fluoxetine may exhibit behavioral changes, affecting their ability to respond to environmental stressors and the interaction with other fish.