Distribution of sodium transporters and aquaporin-1 in the human choroid plexus
Distribution of sodium transporters and aquaporin-1 in the human choroid plexus
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DOI：
10.1152/ajpcell.00433.2005
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发表时间：
2006-07-01
影响因子：
5.5
通讯作者：
Nielsen, Soren
中科院分区：
文献类型：
--
作者：
Praetorius, Jeppe;Nielsen, Soren
脉络丛上皮在脑室腔内高速分泌电解质和液体。一些通道和离子载体已被确定为啮齿动物脉络丛这种运输的可能中介。这项研究的目的是将这些蛋白质中的几个定位于人类脉络丛。用免疫过氧化物酶组织化学方法确定蛋白质在细胞和亚细胞内的定位。水通道，水通道蛋白(AQP)1，主要位于顶端质膜区，尽管也观察到明显的基侧和内皮免疫反应。Na+-K+-ATPaseα(1)-亚基仅定位于人脉络丛上皮细胞顶端。Na+-K+-2Cl(-)共转运体NKCC1的免疫反应同样局限于上皮的顶端质膜区。与依赖于Na+的Cl-/HCO3-交换器NCBE和电子中性型Na+-HCO3-共转运体NBCn1一样，Cl-/HCO3-交换器AE2位于基底面。Na+依赖的酸/碱转运蛋白NHE1或NBCe2未见免疫反应。因此，人脉络丛上皮细胞的水通道和Na+转运体的分布模式与大鼠和小鼠的脉络丛几乎相同。这种一般的跨物种模式表明，这些转运蛋白在脉络丛功能中发挥着中心作用，例如脑脊液的产生。
The choroid plexus epithelium secretes electrolytes and fluid in the brain ventricular lumen at high rates. Several channels and ion carriers have been identified as likely mediators of this transport in rodent choroid plexus. This study aimed to map several of these proteins to the human choroid plexus. Immunoperoxidase-histochemistry was employed to determine the cellular and subcellular localization of the proteins. The water channel, aquaporin (AQP) 1, was predominantly situated in the apical plasma membrane domain, although distinct basolateral and endothelial immunoreactivity was also observed. The Na+-K+-ATPase alpha(1)-subunit was exclusively localized apically in the human choroid plexus epithelial cells. Immunoreactivity for the Na+-K+-2Cl(-) cotransporter, NKCC1, was likewise confined to the apical plasma membrane domain of the epithelium. The Cl-/HCO3- exchanger, AE2, was localized basolaterally, as was the Na+-dependent Cl-/HCO3- exchanger, NCBE, and the electroneutral Na+-HCO3- cotransporter, NBCn1. No immunoreactivity was found toward the Na+-dependent acid/base transporters NHE1 or NBCe2. Hence, the human choroid plexus epithelium displays an almost identical distribution pattern of water channels and Na+ transporters as the rat and mouse choroid plexus. This general cross species pattern suggests central roles for these transporters in choroid plexus functions such as cerebrospinal fluid production.