Effect of chlorogenic acid on intestinal inflammation, antioxidant status, and microbial community of young hens challenged with acute heat stress
Effect of chlorogenic acid on intestinal inflammation, antioxidant status, and microbial community of young hens challenged with acute heat stress
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DOI：
10.1111/asj.13619
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发表时间：
2021-12-01
影响因子：
2
通讯作者：
Wei, Jintao
中科院分区：
文献类型：
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作者：
Chen, Fang;Zhang, Hao;Wei, Jintao
家禽的热应激对生产性能有害。绿原酸（CGA）具有抗菌、抗炎和抗氧化特性。本研究旨在评价日粮中添加CGA对急性热应激雏鸡肠道健康和盲肠菌群组成的影响。试验选用100日龄海兰褐雏，随机分为4组。对照组（C）和热应激组（HS）给予基础日粮。HS + CGA（300）组和HS + CGA（600）组在热应激前分别在基础日粮中添加300和600 mg/kg CGA。HS组、HS + CGA（300）组和HS + CGA（600）组在38 ℃下暴露4 h，而对照组维持在25 ℃下。在本研究中，日粮中添加CGA对急性热应激引起的T-AOC和T-SOD活性下降以及IL-1 β和TNF α的升高有一定的缓解作用。日粮中添加600 mg/kg CGA对提高Rikenellaceae RC9_gut_group、Ruminococcaceae UCG-005和Christensenellaceae R-7_group等有益菌属的相对丰度和降低Sutterella等炎症相关菌属的相对丰度具有较好的效果。因此，CGA可以通过抑制炎症和改善抗氧化能力和盲肠微生物群组成来改善急性热应激损伤。
Heat stress in poultry is deleterious to productive performance. Chlorogenic acid (CGA) exerts antibacterial, anti-inflammatory, and antioxidant properties. This study was conducted to evaluate the effects of dietary supplemental CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups. The control group (C) and heat stress group (HS) received a basal diet. HS + CGA(300) group and HS + CGA(600) group received a basal diet supplemented with 300- and 600-mg/kg CGA, respectively, for 2 weeks before heat stress exposure. Pullets of HS, HS + CGA(300), and HS + CGA(600) group were exposed to 38 degrees C for 4 h while the control group was maintained at 25 degrees C. In this study, dietary CGA supplementation had effect on mitigate the decreased T-AOC and T-SOD activities and the increasing of IL-1 beta and TNF alpha induced by acute heat stress. Dietary supplementation with 600 mg/kg CGA had better effect on increasing the relative abundance of beneficial bacterial genera, such as Rikenellaceae RC9_gut_group, Ruminococcaceae UCG-005, and Christensenellaceae R-7_group, and deceasing bacteria genera involved in inflammation, such as Sutterella species. Therefore, CGA can ameliorate acute heat stress damage through suppressing inflammation and improved antioxidant capacity and cecal microbiota composition.