DNA tube structures controlled by a four-way-branched DNA connector
DNA tube structures controlled by a four-way-branched DNA connector
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DOI：
10.1002/anie.200501034
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发表时间：
2005-01-01
影响因子：
16.6
通讯作者：
Majima, T
中科院分区：
文献类型：
--
作者：
Endo, M;Seeman, NC;Majima, T
硫醇修饰的 10 聚体 DNA 链 (Tc)，与tile BT 的标签链互补（参见支持信息）。将 10 条 DNA 链和 Porph-(Tc) 4 1 混合在一起，并在含有 2-[4-(2-羟乙基)-1-哌嗪基]乙磺酸（HEPES；pH 7.5）、乙二胺四乙酸 (EDTA) 和 Mg2+ 的缓冲液中从 958C 退火至室温 36 小时。 [12]复合物形成后，我们在溶液中使用原子力显微镜（AFM）观察了 DNA 纳米级结构。 [12]在仅使用瓷砖 A 和 BT 退火的情况下，获得的 2D DNA 阵列与之前使用 A-B 阵列系统描述的类似（图 2a）。[6, 7] 相比之下，添加 1/4 的 Porph-(Tc) 4 1 连接器的当量并使用瓷砖 A 和 BT 退火，没有观察到大的 2D 结构，并且出现了纤维状结构（图 2b），长度为达到20μm以上。当相当于 1 的 1/16 用tileA 和 BT 退火时，我们获得了纤维和通常的 2D 阵列的混合物（参见支持信息）。因此，纤维结构的形成取决于瓷砖和 Porph-(Tc) 4 1 之间的化学计量。为了表征详细的纳米级结构，我们分析了 DNA 纤维的表面。 DNA 纤维结构长轴的横截面分析揭示了
thiol-modified 10-mer DNA strand (Tc) that is complementary to the tag strand of the tile BT (see Supporting Information). Ten DNA strands and Porph-(Tc) 4 1 were mixed together and annealed from 958C to room temperature for 36 h in a buffer containing 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid (HEPES; pH 7.5), ethylenediaminetetraacetate (EDTA), and Mg2+.[12] After complex formation, we observed the DNA nanoscale structures by using atomic force microscopy (AFM) in solution.[12] In the case of annealing with tiles A and BT only, 2D DNA arrays were obtained which were similar to those previously described with the A–B array system (Figure 2a).[6, 7] By contrast, with addition of 1/4th of an equivalent of Porph-(Tc) 4 1 connector and annealing with tiles A and BT, the large 2D structures were not observed and fiber-like structures appeared (Figure 2b), the lengths of which reaching over 20 μm. When 1/16th of an equivalent of 1 was annealed with tilesA and BT, we obtained a mixture of fibers and the usual 2D arrays (see Supporting Information). Thus, the formation of fiber structures depended on the stoichiometry between the tiles and Porph-(Tc) 4 1. To characterize the detailed nanoscale structures, we analyzed the surface of the DNA fibers. A cross-section analysis of the long axis of the DNA fiber structure reveals