Laccase detoxification of steam-exploded wheat straw for second generation bioethanol
Laccase detoxification of steam-exploded wheat straw for second generation bioethanol
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DOI：
10.1016/j.biortech.2009.07.049
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发表时间：
2009-12-01
影响因子：
11.4
通讯作者：
Jesus Martinez, Maria
中科院分区：
文献类型：
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作者：
Jurado, Miguel;Prieto, Alicia;Jesus Martinez, Maria
在这项工作中，我们比较了在软条件（水浸渍）和恶劣条件（稀释酸浸渍）下对蒸汽爆炸麦秸进行漆酶处理的效率。分析了几种酶处理参数（pH、孵育时间、漆酶来源和负载）的影响。结果表明，蒸汽爆炸时的严酷条件对脱毒效率有影响。用漆酶聚合游离酚可以降低酚类化合物的毒性作用。漆酶处理对汽爆麦秸酶解后还原糖的回收效果较好，且用纤维素酶水解后效果更好。所有样品经漆酶处理后，水解产物的发酵性都得到了很大的提高。我们的研究结果证明了酚类化合物作为发酵抑制剂的作用，以及漆酶处理在提高蒸汽爆炸麦秸乙醇产量方面的优势。(c) 2009 Elsevier Ltd.版权所有
In this work we compared the efficiency of a laccase treatment performed on steam-exploded wheat straw pretreated under soft conditions (water impregnation) or harsh conditions (impregnation with diluted acid). The effect of several enzymatic treatment parameters (pH, time of incubation, laccase origin and loading) was analysed. The results obtained indicated that severity conditions applied during steam explosion have an influence on the efficiency of detoxification. A reduction of the toxic effect of phenolic compounds by laccase polymerization of free phenols was demonstrated. Laccase treatment of steam-exploded wheat straw reduced sugar recovery after enzymatic hydrolysis, and it should be better performed after hydrolysis with cellulases. The fermentability of hydrolysates was greatly improved by the laccase treatment in all the samples. Our results demonstrate the action of phenolic compounds as fermentation inhibitors, and the advantages of a laccase treatment to increase the ethanol production from steam-exploded wheat straw. (c) 2009 Elsevier Ltd. All rights reserved