Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina
Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina
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DOI：
10.1016/j.ibiod.2015.03.014
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发表时间：
2015-07-01
影响因子：
4.8
通讯作者：
Ferrero, Marcela A.
中科院分区：
文献类型：
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作者：
Isaac, Paula;Martinez, Fabiana L.;Ferrero, Marcela A.
由假单胞菌和放线菌菌株组成的混合培养物显示出有趣的多环芳烃（PAHs）去除值，大大提高了纯培养物中菌株的去除效率。这些细菌混合培养物是用从污染场地分离的细菌合成的，先前在烧瓶规模下操作时对萘、菲和芘的去除进行了评价。结果表明，单培养的假单胞菌P26和假单胞菌N3能有效降解低分子量多环芳烃，但对高分子量多环芳烃的降解能力不强。放线菌Rhodococcus sp. P18、Gordonia sp. H19和Rhodococcus sp. F27对高分子量多环芳烃有较好的降解效果，但对低分子量多环芳烃的降解效果不明显。这五个菌株的组合（称为C15混合培养物）去除100%的萘和菲，并表现出最高的芘生物降解活性，去除值接近42%，几乎6倍以上的纯培养菌株记录的值。此外，这种确定的混合培养物在测试烃的存在下也显示出生物乳化活性，这显著有助于在生物降解过程中溶解它们。我们可以证实，最高芘降解率观察时，生物乳化活性最大的混合培养。我们的研究结果表明，定义的混合文化C15提出了协同活性，有效地去除混合的多环芳烃，提高生物降解过程中的一个有价值的方法。(C)2015爱思唯尔有限公司版权所有。
Defined mixed cultures consisting of Pseudomonas and actinobacteria strains showed interesting polycyclic aromatic hydrocarbons (PAHs) removal values, improving greatly removal efficiency of strains in pure culture. These bacterial mixed cultures were synthesized with bacteria isolated from contaminated sites, previously evaluated regarding of naphthalene, phenanthrene and pyrene removal when operating at flask scale. The results had shown that monocultures of Pseudomonas monteilii P26 and Pseudomonas sp. N3 could degrade efficiently low molecular weight (LMW) PAHs but they did not show interesting high molecular weight (HMW) PAHs removal capabilities. On the other hand, the actinobacteria Rhodococcus sp. P18, Gordonia sp. H19 and Rhodococcus sp. F27 were able to degrade relatively efficiently HMW PAHs but they did not remove LMW PAHs from culture medium. The combination of four of these five strains (called C15 mixed culture) removed 100% of naphthalene and phenanthrene, and showed the highest pyrene biodegradation activity with removal values close to 42%, almost 6-times higher than those values recorded with the strains in pure culture. In addition, this defined mixed culture showed also bioemulsifying activities in presence of the tested hydrocarbons, which notably helps to solubilize them during the biodegradation process. We could confirm that highest pyrene degradation rate was observed when bioemulsifying activity was maximum in the mixed culture. Our results reveal that defined mixed culture C15 presents synergistic activity for effective removal of a mix of PAHs, improving the biodegradation process in a valuable approach. (C) 2015 Elsevier Ltd. All rights reserved.