Alteration in articular cartilage of rat knee joints after spinal cord injury
Alteration in articular cartilage of rat knee joints after spinal cord injury
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DOI：
10.1016/j.joca.2007.07.002
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发表时间：
2008-03-01
影响因子：
7
通讯作者：
Tobimatsu, Y.
中科院分区：
文献类型：
--
作者：
Moriyama, H.;Yoshimura, O.;Tobimatsu, Y.
目的：机械力是维持关节软骨形态和功能完整性的关键。脊髓损伤（SCI）后关节软骨的改变已经被描述为与机械力的抑制有关，因为关节被卸载并限制运动。然而，SCI后软骨的形态学和生化特征仍然知之甚少。我们确定了脊髓损伤后软骨改变的位置，并验证了机械力对关节软骨的影响。共使用32只Wistar大鼠。16只动物接受了SCI，16只动物作为对照。分别在干预后4、8、10、12周通过组织化学、组织形态计量学、免疫组织化学和生化分析对膝关节软骨进行评估。结果：脊髓损伤膝关节软骨厚度在胫骨和股骨后（FP）区域减少，在股骨前（FA）区域增加。脊髓损伤减少了软骨细胞的数量在前面的地区和减少软骨基质染色只在胫骨地区。在浅层中观察到II型胶原的免疫标记，但从中层到深层观察到较弱。I型胶原过度存在于软骨表面和细胞周围regions.Conclusion：脊髓损伤后的软骨改变不会被解释为只有一个抑制的机械力卸载和制动，但可能有影响的软骨除了在机械力的变化。(C)2007年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: Mechanical forces are crucial for the maintenance of the morphologic and functional integrity of articular cartilage. The alteration of the articular cartilage after spinal cord injury (SCI) has been described in relation to a suppression of mechanical forces, since the joint is unloaded and restricted in movement. However, the morphological and biochemical characteristics of the cartilage after SCI are still poorly understood. We identified the localization of cartilage alterations after SCI and verified the influence of mechanical forces on the articular cartilage.Method. A total of 32 Wistar rats were used. Sixteen animals underwent an SCI and 16 animals served as control. The articular cartilage of the knee joint was assessed, respectively, at 4, 8, 10, and 12 weeks after intervention by histochemical, histomorphometric, immunohistochemical, and biochemical analyses.Results: Cartilage thickness of spinal cord-injured knees decreased at the tibial and posterior femoral (FP) regions and increased at the anterior femoral (FA) region. Spinal cord injuries decreased the number of chondrocytes at the anterior regions and decreased the cartilage matrix staining only at the tibial regions. Immunolabeling to collagen type II was noted comparably in the superficial layer but noted weakly from the middle to deep layer. Collagen type I existed excessively at the cartilage surface and the pericellular regions.Conclusion: Cartilage alterations after SCI would not be explained by only a suppression of mechanical forces by unloading and immobilization, but there may be influences on the cartilage in addition to the change in mechanical forces. (C) 2007 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.