SYNTHESIS AND SECRETION OF TRANSFERRIN BY CULTURED MOUSE HEPATOMA-CELLS
SYNTHESIS AND SECRETION OF TRANSFERRIN BY CULTURED MOUSE HEPATOMA-CELLS
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DOI：
10.1111/j.1432-0436.1978.tb00956.x
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发表时间：
1978-01-01
期刊：
影响因子：
2.9
通讯作者：
RAO, EY
中科院分区：
文献类型：
--
作者：
PAPACONSTANTINOU, J;HILL, RE;RAO, EY
组织培养的小鼠肝癌细胞（Hepa-1）已显示合成和分泌三种不同的转铁蛋白。每一种运铁蛋白的分子量都是77，000，这是通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定的。每种形式的浓度由其染色强度表示，其在具有最快移动性的形式中最高，在具有最慢移动性的形式中最低。转铁蛋白合成的相对速率已在对数期和稳定期细胞中测定;数据表明，在稳定期细胞中合成的相对速率增加2倍。当[3 H]亮氨酸掺入转铁蛋白达到稳定状态时，分泌速率等于合成速率;分泌速率在静止期细胞中也增加2倍。我们的研究还表明，转铁蛋白的合成占0.98%的总蛋白质合成的对数期细胞和1.8%的稳定期细胞。这是通过体内研究确定的合成水平。我们的结论是，经过连续培养几年，这些肝癌细胞已保持分化的肝细胞的特征之一，即合成和分泌转铁蛋白的能力。
The mouse hepatoma cell (Hepa-1) in tissue culture has been shown to synthesize and secrete three electrophoretically distinct transferrins. Each of these forms of transferrin has a molecular weight of 77,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The concentration of each form is indicated by its staining intensity, which is highest in the form with the fastest mobility and lowest in the form with the slowest mobility. The relative rate of transferrin synthesis has been determined in log-phase and stationary-phase cells; the data indicate that the relative rate of synthesis increases 2-fold in stationary-phase cells. When the incorporation of [3H]leucine into transferrin reaches steady state, the rate of secretion is equal to the rate of synthesis; the rate of secretion also increases 2-fold in stationary-phase cells. Our studies also show that transferrin synthesis accounts for 0.98% of the total protein synthesis in log-phase cells and for 1.8% in stationary-phase cells. This is the level of synthesis that has been determined by in vivo studies. We conclude that after continuous culture for several years these hepatoma cells have maintained one of the characteristics of the differentiated liver cell, namely, the ability to synthesize and secrete transferrin.