Protein kinase C μ is down-regulated in androgen-independent prostate cancer
Protein kinase C μ is down-regulated in androgen-independent prostate cancer
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DOI：
10.1016/s0006-291x(03)01161-6
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发表时间：
2003-07-25
影响因子：
3.1
通讯作者：
Balaji, KC
中科院分区：
文献类型：
--
作者：
Jaggi, M;Rao, PS;Balaji, KC
进展为雄激素独立（AI）是前列腺癌死亡的主要原因。我们之前通过微阵列分析进行性前列腺癌细胞系模型中的差异基因表达研究发现了前列腺癌中蛋白激酶 C mu (PKCmu) 表达的失调。在这项研究中，定量核糖核酸酶保护测定和免疫印迹分析表明，与其亲本雄激素依赖性（AD）LNCaP前列腺癌细胞相比，AI C4-2细胞中的PKCmu分别在转录和翻译水平上下调。通过体外激酶测定，C4-2 细胞中的 PKCp 激酶活性显着降低。对进展为 AI 前列腺癌的患者的前列腺癌组织进行的免疫组织化学研究表明，在 At 人类前列腺癌中 PKCmu 表达 100% 降低。细胞系模型和人类前列腺癌组织中 PKCmu 的持续下调表明 PKCp 在前列腺癌 AI 进展中可能具有重要的功能作用。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
Progression to androgen independence (AI) is the main cause of death in prostate cancer. Our prior differential gene expression studies by microarray analysis in progressive prostate cancer cell line model identified dysregulation of protein kinase C mu (PKCmu) expression in prostate cancer. In this study, quantitative ribonuclease protection assay and immunoblot analysis demonstrate down regulation of PKCmu at transcription and translational level, respectively, in AI C4-2 cells compared to its parental androgen dependent (AD) LNCaP prostate cancer cells. Significantly lower PKCp kinase activity was confirmed in C4-2 cells by in vitro kinase assay. Immunohistochemical studies of prostate cancer tissue from patient progressing to AI prostate cancer demonstrated that PKCmu expression is decreased in 100% of At human prostate cancers. The consistent down regulation of PKCmu in cell line models and human prostate cancer tissues suggests a possible functionally significant role for PKCp in progression to AI in prostate cancer. (C) 2003 Elsevier Science (USA). All rights reserved.