Local Delivery of Growth Factors Using Coated Suture Material
Local Delivery of Growth Factors Using Coated Suture Material
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DOI：
10.1100/2012/109216
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Schmidmaier, G.
中科院分区：
文献类型：
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作者：
Fuchs, T. F.;Surke, C.;Schmidmaier, G.
各种组织中愈合过程的优化是外科研究的中心点。一种方法是用生长因子刺激愈合过程。这些物质的半衰期很短，因此局部施用这些物质似乎比全身施用更有用。一种可能的局部递送方法是将生长因子掺入生物可吸收的聚（D，L-丙交酯）悬浮液（PDLLA）并涂覆缝合材料。本研究的目的是建立一种使用涂层缝合材料局部输送生长因子的程序。在动物模型中测试了涂有生长因子的缝线。使用单丝缝合线在大鼠模型中创建结肠吻合术。它们要么未经处理，要么单独涂有 PDLLA 涂层，或者涂有掺有胰岛素样生长因子-I (IGF-I) 的 PDLLA。对吻合进行生物力学、组织学和免疫组织化学检查。 3天后，治疗组显示出比对照组明显更强的承受生物力学压力的能力。这一发现得到了组织形态计量学结果的支持。研究结果表明，使用 PDLLA 涂层缝合材料可以局部输送生物活性生长因子。因此，可以局部刺激愈合过程，而不会使整个有机体遭受潜在破坏性的高全身剂量。
The optimization of healing processes in a wide range of tissues represents a central point for surgical research. One approach is to stimulate healing processes with growth factors. These substances have a short half-life and therefore it seems useful to administer these substances locally rather than systemically. One possible method of local delivery is to incorporate growth factors into a bioabsorbable poly (D, L-lactide) suspension (PDLLA) and coat suture material. The aim of the present study was to establish a procedure for the local delivery of growth factors using coated suture material. Sutures coated with growth factors were tested in an animal model. Anastomoses of the colon were created in a rat model using monofilament sutures. These were either untreated or coated with PDLLA coating alone or coated with PDLLA incorporating insulin-like growth factor-I (IGF-I). The anastomoses were subjected to biomechanical, histological, and immunohistochemical examination. After 3 days the treated groups showed a significantly greater capacity to withstand biomechanical stress than the control groups. This finding was supported by the results of the histomorphometric. The results of the study indicate that it is possible to deliver bioactive growth factors locally using PDLLA coated suture material. Healing processes can thus be stimulated locally without subjecting the whole organism to potentially damaging high systemic doses.