Disruption of a phytochrome-like histidine kinase gene by homologous recombination leads to a significant reduction in vegetative growth, sclerotia production, and the pathogenicity of Botrytis cinerea
Disruption of a phytochrome-like histidine kinase gene by homologous recombination leads to a significant reduction in vegetative growth, sclerotia production, and the pathogenicity of Botrytis cinerea
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通过同源重组破坏光敏色素样组氨酸激酶基因会导致灰葡萄孢的营养生长、菌核产生和致病性显着降低
DOI：
10.1016/j.pmpp.2013.12.002
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发表时间：
2014-01-01
影响因子：
2.7
通讯作者：
Xu, Ling
中科院分区：
文献类型：
--
作者：
Hu, Youzhen;He, Jinyan;Xu, Ling
光敏色素样组氨酸激酶是一种光感受器，帮助各种细菌和真菌感知环境光。在本研究中，我们从灰霉病菌中克隆了一个可能的组氨酸激酶基因BcPhy3，并获得了一个BcPhy3突变的Delta BcPhy3-P21。与野生型菌株Bc05.10相比，该突变株在PDA上形成的菌落较小(约为Bc05.10的70%)，表明BcPhy3的缺失影响了Delta BcPhy3-P21的生长速度。与Bc05.10相比，该突变体产生的菌核数量也大大减少。此外，Delta BcPhy3-P21菌株对辣椒、生菜、番茄、葡萄浆果和胡萝卜等不同植物组织的致病力显著降低。进一步的表型分析表明，Delta BcPhy3-P21突变体对刚果红引起的细胞壁胁迫更敏感，这表明BcPhy3的缺失也可能影响细胞壁的完整性。透射电子显微镜分析进一步证实，Delta-P21的细胞壁层较薄，主要由甲壳素组成，外层细胞壁结构紊乱。基因转录分析表明，几丁质合成基因(Bcchs1和Bcchs3a)在Delta BcPhy3-P21中的表达受到抑制。几丁质含量的测定表明，与野生型菌株Bc05.10相比，Delta BcPhy3突变体的菌丝体几丁质含量降低了约19%。综上所述，我们的结果表明，编码组氨酸激酶的BcPhy3是灰霉病菌正常营养生长、菌核产生和致病所必需的。(C)2013爱思唯尔有限公司。保留所有权利。
Phytochrome-like histidine kinases are photoreceptors that help a wide variety of bacteria and fungi perceive environmental light. In this study, a putative histidine kinase gene Bcphy3 from Botrytis cinerea was disrupted, and a Bcphy3 mutan Delta Bcphy3-P21 was obtained. This mutant formed smaller colonies on PDA media compared to the wild-type strain Bc05.10 (approximately 70% of Bc05.10), indicating that the absence of Bcphy3 affected the growth rate of Delta Bcphy3-P21. The mutant also produced a substantially reduced amount of sclerotia compared to Bc05.10. Moreover, the Delta Bcphy3-P21 strain displayed significantly reduced pathogenicity on different tested plant tissues (capsicum, lettuce, tomato, grape berry, and carrot). Further phenotypic analysis showed that the Delta Bcphy3-P21 mutant was more susceptible to cell wall stress caused by Congo red, suggesting that the absence of Bcphy3 may also affect cell wall integrity. Transmission electron microscopy analysis further confirmed that the layer of cell wall mainly composed of chitin was much thinner, and the outer layer of cell wall was disorganized in Delta-P21. Gene transcription analysis demonstrated that the expression of the chitin synthesis genes (Bcchs1 and Bcchs3a) was inhibited in Delta Bcphy3-P21. Determination of the chitin content revealed that the chitin content of the mycelium in the Delta Bcphy3 mutant was reduced by approximately 19% compared to the wild-type strain Bc05.10. Taken together, our results indicated that Bcphy3 encoding a histidine kinase is required for normal vegetative growth, sclerotia production, and pathogenicity in B.cinerea. (C) 2013 Elsevier Ltd. All rights reserved.