TEMPORAL EXPRESSION OF MYOGENIC REGULATORY GENES DURING ACTIVATION, PROLIFERATION, AND DIFFERENTIATION OF RAT SKELETAL-MUSCLE SATELLITE CELLS
TEMPORAL EXPRESSION OF MYOGENIC REGULATORY GENES DURING ACTIVATION, PROLIFERATION, AND DIFFERENTIATION OF RAT SKELETAL-MUSCLE SATELLITE CELLS
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DOI：
10.1002/jcp.1041590222
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发表时间：
1994-05-01
影响因子：
5.6
通讯作者：
ALLEN, RE
中科院分区：
文献类型：
--
作者：
SMITH, CK;JANNEY, MJ;ALLEN, RE
卫星细胞负责出生后骨骼肌的生长和修复。我们研究了这些细胞从静止到融合阶段的肌源性调节基因（MRG）家族的表达。通过聚合酶链反应扩增从成年大鼠卫星细胞中分离出的逆转录RNA（RT-PCR），我们证明了基因激活的时间序列，这与之前在胚胎闪族细胞中观察到的不同。在主要静止细胞中未检测到 MRG 表达。在细胞培养中，MyoD 在首次出现增殖证据之前 12 小时被激活。 MRF4 和 myf-5 在 48 小时出现，可能与第一个分裂周期相关。直到卫星细胞从肌纤维中恢复后 72 小时才能检测到肌细胞生成素，这与第一个分化证据一致。 (C) 1994 年 Wiley-Liss 公司
The satellite cell is responsible for growth and repair of postnatal skeletal muscle. We investigated the expression of the myogenic regulatory gene (MRG) family in these cells in the stages from quiescence to fusion. Using polymerase chain reaction amplification of reverse-transcribed RNA (RT-PCR) isolated from adult rat satellite cells, we demonstrated a temporal sequence of gene activation, which is distinct from that previously observed in embryonic semitic cells. No MRG expression was detected in predominantly quiescent cells. MyoD is activated by 12 h in cell culture, prior to the first evidence of proliferation. MRF4 and myf-5 appear by 48 h and may be associated with the first division cycle. Myogenin is not detectable until 72 h after satellite cell recovery from the muscle fiber, coincidental with the first evidence of differentiation. (C) 1994 Wiley-Liss, Inc.