Effect of leptin on hypothalamic gene expression in calorie-restricted rats
Effect of leptin on hypothalamic gene expression in calorie-restricted rats
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DOI：
10.1093/gerona/61.9.890
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发表时间：
2006-09-01
影响因子：
5.1
通讯作者：
Shimokawa, Isao
中科院分区：
文献类型：
--
作者：
Komatsu, Toshimitsu;Chiba, Takuya;Shimokawa, Isao
下丘脑弓状核 (ARH) 的瘦素信号减弱可能会诱导热量限制 (CR) 特异性的神经内分泌和代谢适应，这可能与 CR 的作用相关。本研究调查了瘦素向 ARH 信号传导的恢复是否可以逆转 CR 诱导的大鼠神经肽基因表达的改变。雄性 F344 大鼠从 6 周龄起随意喂养或 30% CR 饮食，在 34 周龄时通过植入皮下组织的渗透微型泵在脑室内接受瘦素或载体 14 天。使用从含有 ARH 的显微解剖组织中提取的总 RNA，通过实时逆转录聚合酶链反应对信使 RNA 水平进行定量。结果表明，瘦素给药逆转了 CR 大鼠中神经肽 Y 和刺鼠相关蛋白基因的上调表达，表明瘦素-ARH 通路可能在 CR 效应中发挥作用。
Diminished leptin signaling to the arcuate nucleus of hypothalamus (ARH) may induce calorie restriction (CR)-specific neuroendocrine and metabolic adaptation, which is potentially relevant to the effect of CR. The present study investigated whether restoration of leptin signaling to the ARH could reverse CR-induced alterations in neuropeptide gene expression in rats. Male F344 rats, fed ad libitum or a 30% CR diet from 6 weeks of age, received leptin or vehicle intracerebroventricularly for 14 days via osmotic mini-pumps implanted in the subcutis at 34 weeks of age. The messenger RNA levels were quantified by real-time reverse transcription-polymerase chain reaction using total RNA extracted from microdissected tissues containing the ARH. The results indicated that leptin administration reversed the upregulated expression of neuropeptide Y and agouti-related protein genes in CR rats, suggesting the possibility of a role for the leptin-ARH pathway in the effect of CR.