THERMAL PERTURBATION DIFFERENTIAL SPECTRA OF RIBONUCLEIC-ACIDS .2. NEAREST NEIGHBOR INTERACTIONS
THERMAL PERTURBATION DIFFERENTIAL SPECTRA OF RIBONUCLEIC-ACIDS .2. NEAREST NEIGHBOR INTERACTIONS
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DOI：
10.1093/nar/7.7.1981
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发表时间：
1979-01-01
影响因子：
14.9
通讯作者：
GABARROARPA, J
中科院分区：
文献类型：
--
作者：
FRECHET, D;EHRLICH, R;GABARROARPA, J
此处使用二核苷一磷酸作为模型来研究与碱基堆积相关的减色效应的序列依赖性。结果表明，一旦从二核苷一磷酸的热扰动差异光谱中减去由于碱基的温度依赖性水合变化而产生的贡献，就可以获得仅由于碱基的解堆积而导致的二聚体的吸光度变化。为了能够使用这些校正的热扰动差异光谱作为研究核酸中最近邻相互作用的模型，有必要将它们标准化为100%碱基解堆积。为了进行这种标准化，通过二态模型从校正的转变曲线中提取表观热力学参数。
Dinucleoside monophosphates are used here as models for studying sequence dependence of the hypochromic effect correlated with base stacking. It was shown that once the contribution due to the temperature dependent hydration change of the bases is substracted from the thermal perturbation difference spectra of dinucleoside monophosphates, the absorbance change of the dimer only due to unstacking of the bases could be obtained. In order to be able to use these corrected thermal perturbation difference spectra as models for studying nearest neighbour interactions in nucleic acids, it was necessary to normalize them to 100% unstacking of the bases.To perform this normalization, apparent thermodynamic parameters were extracted from the corrected transition curves by means of the two-state model.