Phylogenomics of the tropical plant family Ochnaceae using targeted enrichment of nuclear genes and 250+ taxa
Phylogenomics of the tropical plant family Ochnaceae using targeted enrichment of nuclear genes and 250+ taxa
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使用核基因和 250 个分类单元的定向富集对热带植物科 Ochnaceae 进行系统发育分析
作者：
Schneider;Jungcurt;Cardoso;Amorim;Töpel;Andermann;Berberich
对核基因的定向捕获越来越有助于解开迄今为止仍未解决的系统发育关系，因为传统的桑格测序的局限性。特别是，热带植物科的研究受到了损害，因为它们往往依赖于从植物标本中获得的高度降解的DNA。一个这样的例子是泛热带菊科植物，它由33个属和大约550种组成，主要分布在热带稀树草原和潮湿的热带森林中。在这里，我们开发了一套诱饵，覆盖了275个核基因的约660,000个 碱基，用于靶向浓缩，除一个属和250多个物种外。利用这个新的数据集，我们解析了包括奥奇尼亚科在内的奥奇纳科的系统发育骨架，并建立了新的关系。最重要的是，我们的发现突出了新热带和古热带的分类群形成了独立的支系，需要重新建立以前分开的属。Ouratea和Ochna(都是Ochniae)是到目前为止物种最丰富的属，分别有120种和41种。相反，物种丰富的第三大属--空肠弯曲属是两个不同支系的多系分类。Ouratea是奥奇尼亚科唯一的新热带属，是这个亚族的五个古热带属的姐妹。这项研究中开发的诱饵试剂盒被证明对解开奥奇尼亚科之间的关系特别有用，奥奇尼亚科包括该科约三分之二的物种多样性。
Targeted capture of nuclear genes increasingly contributes to unravelling phylogenetic relationships that hitherto remained unresolved because of limitations of traditional Sanger sequencing. In particular, the study of tropical plant families has been compromised because they often rely on highly degraded DNA obtained from herbarium specimens. One such example is the pantropical Ochnaceae, which comprises 33 genera and approximately 550 species, occurring mostly in savannas and moist tropical forests. Here, we developed a set of baits covering about 660,000 bp from 275 nuclear genes used for the targeted enrichment based on all but one genus and more than 250 species. Using this novel dataset, we resolved the phylogenetic backbone of Ochnaceae, including that of Ochninae, and we established new relationships. Most importantly, our findings highlight that the neotropical and palaeotropical taxa ofSauvagesiaform independent clades, requiring the re‐erection of formerly separate genera.OurateaandOchna(both Ochninae), by far the most species‐rich genera and represented by 120 and 41 species, respectively, came out as monophyletic. In contrast, the third‐most species‐rich genus,Campylospermum, is polyphyletic in two distinct clades.Ouratea, the only neotropical genus of Ochninae, was sister to the five palaeotropical genera of this subtribe. The bait kit developed in this study proved to be particularly useful for unravelling relationships within Ochninae, which includes about two‐thirds of the species diversity in the family.
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Recherches sur les Ochnacées：II。
DOI：
--
发表时间：
1970
期刊：
影响因子：
--
作者：
C. Sastre
通讯作者：
C. Sastre
解开Mata Atlantica中巨型多样性类群的属下分类：Miconia部分Chaenanthera（Melatomataceae：Miconieae）的系统发育
影响因子：
3.4
作者：
R. Goldenberg;M. Reginato;F. Michelangeli
通讯作者：
F. Michelangeli
布雷西尔新奥赫纳西斯
DOI：
--
发表时间：
1981
期刊：
影响因子：
--
作者：
C. Sastre
通讯作者：
C. Sastre
不寻常且分布狭窄的Rhytidanthera属（Ochnaceae）的系统发育位置和腺体结构的进化
DOI：
--
发表时间：
2020
期刊：
影响因子：
--
作者：
Sandra Reinales;C. Parra‐O.
通讯作者：
C. Parra‐O.
影响因子：
3.3
作者：
Oliver, Jeffrey C.
通讯作者：
Oliver, Jeffrey C.