Olfactory coding of intra- and interspecific pheromonal messages by the male Mythimna separata in North China
Olfactory coding of intra- and interspecific pheromonal messages by the male Mythimna separata in North China
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华北雄性粘虫种内和种间信息素信息的嗅觉编码
DOI：
10.1016/j.ibmb.2020.103439
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发表时间：
2020-10-01
影响因子：
3.8
通讯作者：
Wang, Chen-Zhu
中科院分区：
文献类型：
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作者：
Jiang, Nan-Ji;Tang, Rui;Wang, Chen-Zhu
飞蛾经常使用固定比例的多组分信息素来保持种内交流和种间隔离。不同寻常的是，中国北方的东方粘虫（Mythimna separata）只使用Z11-16:Ald作为其性信息素的基本成分来寻找配偶。为了了解该物种是如何与其他物种保持行为隔离的，我们研究了雄性分离木的种内和种间信息素信息的嗅觉编码。首先，通过单感器记录对雄触角长毛状感器进行了功能表征。A型感受器对Z11-16:Ald和Z9-14:Ald有应答，B型感受器主要对Z9-14:Ald有应答，对Z11-16:Ac、Z11-16:OH和Z9-16:Ald也有应答。接下来，我们通过体内光学成像检查了触角叶的肾小球对这些化合物的反应。结果表明，在大肾小球复合物（MGC）的3个亚基中，Z11-16:Ald激活积云，Z9-14:Ald激活背前和积云，Z11-16:OH和Z11-16:Ac分别激活背前和背后。然而，Z9-16:Ald激活了普通肾小球。第三，我们在风洞中测试了雄性对这些化合物的行为反应。以1:10的比例添加Z9-14:Ald大大降低了Z11-16:Ald的吸引力，以1:1的比例添加Z9-16:Ald或Z11-16:OH也有行为拮抗作用，而添加Z11-16:Ac对Z11-16:Ald的吸引力没有影响。最后，我们利用触角转录组数据和爪蟾表达系统鉴定了分离田鼠的Z9-14:Ald受体。共表达MsepOR2和MsepORco的爪蟾卵母细胞对Z9-14:Ald有较强的应答。双色荧光原位杂交证实表达MsepOR2和MsepOR3的细胞分别为Z9-14:Ald和Z11-16:Ald，定位于雄触角的不同感受器中。通过比较相关物种的性信息素交流通道，我们的研究结果表明，行为拮抗剂的保守嗅觉通路在夜蛾物种的行为隔离中起着至关重要的作用。
Moths often use multi-component pheromones with fixed ratios to keep intraspecific communication and interspecific isolation. Unusually, the Oriental armyworm Mythimna separata in North China use only Z11-16:Ald as the essential component of its sex pheromone to find mates. To understand how this species keeps behavioral isolation from other species sharing Z11-16:Ald as a major pheromone component, we study the olfactory coding of intra- and interspecific pheromonal messages in the males of M. separata. Firstly, we functionally characterized the long trichoid sensilla in male antennae by single sensillum recording. Two types of sensilla were classified: the A type sensilla responded to Z11-16:Ald and Z9-14:Ald, and the B type sensilla mainly to Z9-14:Ald, and also to Z11-16:Ac, Z11-16:OH, and Z9-16:Ald. Next, we examined the glomerulus responses in the antennal lobes to these compounds by using in vivo optical imaging. The results showed that among the three subunits of the macroglomerular complex (MGC), Z11-16:Ald activated the cumulus, Z9-14:Ald activated the dorso-anterior and the cumulus, Z11-16:OH and Z11-16:Ac activated the dorso-anterior and dorso-posterior, respectively. However, Z9-16:Ald activated an ordinary glomerulus. Thirdly, we tested the behavioral responses of the males to these compounds in the wind tunnel. Addition of Z9-14:Ald at the ratio of 1:10 greatly reduced the attractiveness of Z11-16:Ald, addition of Z9-16:Ald or Z11-16:OH at the ratio of 1:1 also had behavioral antagonistic effects, while addition of Z11-16:Ac had no effect on the attractiveness of Z11-16:Ald. Finally, we used antennal transcriptome data and the Xenopus expression system to identify the receptor of Z9-14:Ald in M. separata. The Xenopus oocytes co-expressing MsepOR2 and MsepORco showed a strong response to Z9-14:Ald. Two-color fluorescence in situ hybridization validated that the cells expressing MsepOR2 and MsepOR3, tuned to Z9-14:Ald and Z11-16:Ald respectively, were localized in the different sensilla of male antennae. Comparing the sex pheromone communication channel of the related species, our results suggest that the conserved olfactory pathways for behavioral antagonists play a crucial role in behavioral isolation of noctuid species.