Combined low-dose LiCl and LY294002 for the treatment of osteoporosis in ovariectomized rats
Combined low-dose LiCl and LY294002 for the treatment of osteoporosis in ovariectomized rats
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DOI：
10.1186/s13018-019-1210-1
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发表时间：
2019-06-13
影响因子：
2.6
通讯作者：
Sun, Guicai
中科院分区：
文献类型：
--
作者：
Bai, Jianhai;Xu, Yier;Sun, Guicai
BackgroundTo provide a low-toxicity and high-efficacy clinical treatment for osteoporosis via a novel combination of LiCl and LY294002.MethodsThe protein levels of p-AKT, AKT, p-GSK3, GSK3, -catenin, p--catenin, and NFATC1 were measured in osteoblasts and osteoclasts by Western blot. ALP activity and TRACP activity were measured using the corresponding kit. The levels of BALP, PINP, CTX, and TRACP-5b were determined in accordance with the requirements of the ELISA kits. Microstructural analysis was performed on the left distal femur using microcomputed tomography.ResultsTreatment with the combination of LiCl and LY294002 led to a markedly increased osteoblast activity but significantly decreased osteoclast differentiation and bone absorption capacity compared with the treatment with LiCl or LY294002 alone (P
