An interaction of the functionalized closo-borates with albumins: The protein fluorescence quenching and calorimetry study
An interaction of the functionalized closo-borates with albumins: The protein fluorescence quenching and calorimetry study
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DOI：
10.1016/j.jlumin.2015.08.042
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发表时间：
2016-01-01
影响因子：
3.6
通讯作者：
Elskaya, Anna V.
中科院分区：
文献类型：
--
作者：
Losytskyy, Mykhaylo Yu.;Kovalska, Vladyslava B.;Elskaya, Anna V.
研究了靠近硼酸盐K-2[B10H10]、K-2[B12H12]及其功能化衍生物与血清蛋白(人（RSA）、牛（BSA）白蛋白、免疫球蛋白IgG、球状蛋白-乳球蛋白和溶菌酶的相互作用。稳态和时间分辨蛋白质荧光猝灭研究指出了近硼酸芳胺衍生物与血清白蛋白的结合和对其他蛋白质的识别。提出了近硼酸芳胺衍生物对白蛋白荧光猝灭的机理。等温滴定量热法（ITC）证实了白蛋白与近硼酸盐分子之间的络合物形成。该化合物（K-2[B10H10]）及其芳胺衍生物均与HSA相互作用，其K-a值（分别为1.4和1.2 × 10(3) M-1）和吉布斯能（分别为-17.9和-17.5 kJ/mol）相近。然而，与母体近硼酸盐（2:1）相比，芳胺衍生物形成的配合物具有更高的客体/宿主结合比（4:1）。(C) 2015 Elsevier B.V.版权所有
An interaction of the boron clusters closo-borates K-2[B10H10], K-2[B12H12] and their functionalized derivatives with serum proteins human (RSA) and bovine (BSA) albumins and immonoglobulin IgG as well as globular proteins beta-lactoglobulin and lysozyme was characterized. The steady state and time resolved protein fluorescence quenching studies point on the binding of the closo-borate arylamine derivatives to serum albumins and discrimination of other proteins. The mechanism of the albumin fluorescence quenching by the closo-borate arylamine derivatives was proposed.The complex formation between albumin and the closo-borate molecules has been confirmed by isothermal titration calorimetry (ITC). The compound (K-2[B10H10]) and its arylamine derivative both interact with HSA, have close values of K-a (1.4 and 1.2 x 10(3) M-1 respectively) and Gibbs energy (-17.9 and -17.5 kJ/mol respectively). However, the arylamine derivative forms complex with the higher guest/host binding ratio (4:1) comparing to the parent closo-borate (2:1). (C) 2015 Elsevier B.V. All rights reserved.