Bmp signaling promotes intermediate mesoderm gene expression in a dose-dependent, cell-autonomous and translation-dependent manner
Bmp signaling promotes intermediate mesoderm gene expression in a dose-dependent, cell-autonomous and translation-dependent manner
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DOI：
10.1016/j.ydbio.2005.09.025
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发表时间：
2005-12-01
影响因子：
2.7
通讯作者：
Schultheiss, TM
中科院分区：
文献类型：
--
作者：
James, RG;Schultheiss, TM
中间中胚层位于体节和侧板之间，是发育中的脊椎动物胚胎中所有肾组织的来源。虽然已知骨形态发生蛋白（Bmp）信号传导调节中胚层细胞类型确定沿着中-外侧轴，但其在中间中胚层形成中的作用尚未得到很好的表征。目前的研究发现，低水平和高水平的BMP配体都是必要的和足够的激活中间和外侧中胚层基因的表达，分别在体内和体外。剂量依赖性激活的中间和侧面的中胚层基因的BMP信号是细胞自主的，证明了由不同强度的启动子驱动的组成型活性BMP受体的禽胚胎的电穿孔。在外植体培养物中，BMP激活的Odd-跳过相关1（Odd-1），最早的已知基因在中间中胚层中表达，被环己酰胺，表明激活Odd-1的BMP信号是抑制依赖性的。本研究的数据与其他研究的数据相结合，以产生一个模型的作用，BMP信号在躯干中胚层图案，其中低水平的BMP激活中间中胚层基因表达的抑制存在于中间中胚层，而高水平的BMP抑制中间和中间中胚层基因表达和激活侧板基因。(C)2005年爱思唯尔公司All rights reserved.
The intermediate mesoderm lies between the somites and the lateral plate and is the source of all kidney tissue in the developing vertebrate embryo. While bone morphogenetic protein (Bmp) signaling is known to regulate mesodermal cell type determination along the medio-lateral axis, its role in intermediate mesoderm formation has not been well characterized. The current study finds that low and high levels of Bmp ligand are both necessary and sufficient to activate intermediate and lateral mesodermal gene expression, respectively, both in vivo and in vitro. Dose-dependent activation of intermediate and lateral mesodermal genes by Bmp signaling is cell-autonomous, as demonstrated by electroporation of the avian embryo with constitutively active Bmp receptors driven by promoters of varying strengths. In explant cultures, Bmp activation of Odd-skipped related 1 (Odd-1), the earliest known gene expressed in the intermediate mesoderm, is blocked by cyclohexamide, indicating that the activation of Odd-1 by Bmp signaling is translation-dependent. The data from this study are integrated with that of other studies to generate a model for the role of Bmp signaling in trunk mesodermal patterning in which low levels of Bmp activate intermediate mesoderm gene expression by inhibition of repressors present in medial mesoderm, whereas high levels of Bmp repress both medial and intermediate mesoderm gene expression and activate lateral plate genes. (C) 2005 Elsevier Inc. All rights reserved.