Characterization of the Expression Pattern of the PRC2 Core Subunit Suz12 During Embryonic Development of Xenopus Laevis
Characterization of the Expression Pattern of the PRC2 Core Subunit Suz12 During Embryonic Development of Xenopus Laevis
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DOI：
10.1002/dvdy.22120
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发表时间：
2009-12-01
影响因子：
2.5
通讯作者：
Vetter, Monica L.
中科院分区：
文献类型：
--
作者：
Aldiri, Issam;Vetter, Monica L.
Polycomb抑制复合体2是一种多聚体聚集体，可介导多种基因的沉默，并与干细胞维持和癌症进展等重要生物学背景相关。PRC2主要三甲基化组蛋白H3的赖氨酸27，由三个核心亚基组成：EZH2、EED和SUZ12。爪蟾PRC2亚基Ezh2和Eed的直系同源物已被描述，但Suz12仍未确定。本文报道了非洲爪蟾Suz12的克隆，并测定了其发育过程中的时空表达。Xsuz12转录本由母体提供，并在整个发育过程中持续表达，特别是在发育中的中枢神经系统的前部。重要的是，对PRC2亚基Xez、Xeed和Xrbbp4表达的比较分析表明，它们在神经系统中的表达与Xsuz12基本一致，这表明PRC2可能在青蛙中枢神经系统的发育中具有未被探索的功能。科学通报，2009(4):387 - 387。(C) 2009 Wiley-Liss, Inc。
The Polycomb repressive complex 2 is a multimeric aggregate that mediates silencing of a broad range of genes, and is associated with important biological contexts such as stem cell maintenance and cancer progression. PRC2 mainly trimethylates lysine 27 of histone H3 and is composed of three essential core subunits: EZH2, EED, and SUZ12. The Xenopus orthologs of PRC2 subunits Ezh2 and Eed have been described but Suz12 remained unidentified. Here, we report the cloning of the Xenopus Suz12, and determine its spatiotemporal expression during development. Xsuz12 transcript is provided maternally and continues to be expressed throughout development, particularly in the anterior part of the developing central nervous system. Importantly, comparative analysis of the expression of the PRC2 subunits Xez, Xeed, and Xrbbp4 indicates that their expression largely coincides with Xsuz12 in the nervous system, suggesting that PRC2 may have unexplored functions in the development of the frog central nervous system. Developmental Dynamics 238:3185-3192, 2009. (C) 2009 Wiley-Liss, Inc.