Achieving high survival rate following cryopreservation after isolation of prepubertal mouse spermatogonial cells
Achieving high survival rate following cryopreservation after isolation of prepubertal mouse spermatogonial cells
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DOI：
10.1007/s10815-009-9298-6
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发表时间：
2009-03-01
影响因子：
3.1
通讯作者：
Panah, Mehdi Lotfi
中科院分区：
文献类型：
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作者：
Alipoor, Firooz Jannat;Gilani, Mohammad Ali Sadighi;Panah, Mehdi Lotfi
采用磁激活细胞分选法(MACS)分离精原细胞，分别用抗CD49f(α6整合素)抗体和DYNABEADS冻存于含50%、60%和70%三种不同FBS浓度的DMSO冻存液中，平均(+/-SD)纯度为92.52+/-3.57(92.43-98.25)。冻存组I、II、III细胞存活率分别为39.60+/-1.48(37.98~41.62)、89.05+/-3.83(80.83~90.33)和90.52+/-1.71(89.07~92.52)，使用α6整合素和磁珠可获得较高的活细胞数和纯度。精原细胞解冻后，在含60%胎牛血清的冻存液中存活率最高。
Isolating spermatogonia cells with high purity and viability and achieving better survival rate following cryopreservationIsolating the cells by Magnetic Activating Cell Sorting (MACS) method using anti CD49f (alpha 6 integrin) antibody and Dynabeads and freezing in DMSO-based freezing mediums containing three different FBS concentrations of 50%, 60% and 70%.The mean (+/- SD) purity of the isolated cells was 92.52 +/- 3.57 (range 92.43-98.25). The cells frozen in group I, II and III had mean 39.60 +/- 1.48 (range 37.98-41.62), 89.05 +/- 3.83 (range 80.83-90.33) and 90.52 +/- 1.71 (range 89.07-92.52) viability, respectively.Higher viable cell counts and purity can be attained by the use of alpha 6 integrin and magnetic beads. After the thawing of spermatogonial cells, optimum viability was achieved in freezing media containing 60% FBS.