A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000
A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000
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DOI：
10.1094/mpmi-22-1-0052
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发表时间：
2009-01-01
影响因子：
3.5
通讯作者：
Vinatzer, Boris A.
中科院分区：
文献类型：
--
作者：
Almeida, Nalvo F.;Yan, Shuangchun;Vinatzer, Boris A.
多种基因产物，包括植物毒素、病原体相关分子模式和 III 型分泌效应子，影响丁香假单胞菌菌株和植物之间的相互作用，其他尚未表征的因素也可能有所贡献。特别令人感兴趣的是那些控制病原体-宿主特异性的相互作用。具有不同宿主特异性的密切相关病原体的比较基因组学代表了鉴定有助于确定宿主范围的基因的极好方法。丁香假单胞菌 pv. 的基因组序列草案。为此目的获得了对番茄致病但对拟南芥非致病的番茄T1，并与密切相关的拟南芥和番茄模型病原体丁香假单胞菌的基因组进行了比较。番茄DC3000。尽管两个基因组的总体遗传内容似乎高度相似，但效应子的组成却存在显着差异。几种丁香假单胞菌 pv。菌株 DC3000 中缺失的番茄 T1 效应子被证实可转移到植物中，经过充分研究的效应子 AvrRpt2 代表了确定宿主范围的可能候选者。然而，avrRpt2 的存在不足以解释拟南芥对丁香假单胞菌的抗性。番茄 T1，表明其他效应器和可能的 III 型分泌系统独立因素也在这种相互作用中发挥作用。
Diverse gene products including phytotoxins, pathogen-associated molecular patterns, and type III secreted effectors influence interactions between Pseudomonas syringae strains and plants, with additional yet uncharacterized factors likely contributing as well. Of particular interest are those interactions governing pathogen-host specificity. Comparative genomics of closely related pathogens with different host specificity represents an excellent approach for identification of genes contributing to host-range determination. A draft genome sequence of Pseudomonas syringae pv. tomato T1, which is pathogenic on tomato but nonpathogenic on Arabidopsis thaliana, was obtained for this purpose and compared with the genome of the closely related A. thaliana and tomato model pathogen P. syringae pv. tomato DC3000. Although the overall genetic content of each of the two genomes appears to be highly similar, the repertoire of effectors was found to diverge significantly. Several P. syringae pv. tomato T1 effectors absent from strain DC3000 were confirmed to be translocated into plants, with the well-studied effector AvrRpt2 representing a likely candidate for host-range determination. However, the presence of avrRpt2 was not found sufficient to explain A. thaliana resistance to P. syringae pv. tomato T1, suggesting that other effectors and possibly type III secretion system-independent factors also play a role in this interaction.