Ontogeny of dry gas hyperpnea-induced bronchoconstriction in guinea pigs.
Ontogeny of dry gas hyperpnea-induced bronchoconstriction in guinea pigs.
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豚鼠干气过度呼吸引起的支气管收缩的个体发育。
DOI：
10.1152/jappl.1994.76.3.1150
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Solway,J
中科院分区：
文献类型：
--
作者：
Murphy,TM;Ray,DW;Alger,LE;Phillips,IJ;Roach,JC;Leff,AR;Solway,J
青春期豚鼠(AGPs)表现出干气高呼吸诱导的支气管收缩(HIB)，在患有哮喘的人类中与HIB具有相同的关键特征。未成熟动物的呼吸道对不同类型的刺激表现出更强的反应性。我们测试了干气HIB在新生豚鼠(NGP)中是否也增加。我们将HIB量化为5个4-7天大的NGP在高呼吸10min后呼吸系统阻力(RRS)比基线(BL)增加的分数，以及静脉注射乙酰甲胆碱或辣椒素引起的RRS的变化，并将这些反应与AGP的反应进行比较。在高呼吸期间，通过在3.0、4.5和6.0倍安静的真呼吸分钟(VE)时机械地施加高呼吸来提供类似的刺激。在AGPS中，高呼吸引起明显的支气管收缩，并随着VE的增加而增加；RRS的峰值分数增加是BL的7.6+/-2.0倍。相反，高呼吸对NGP无明显的支气管收缩作用(最大VE后1.4+/-0.2倍的BL值；P<；0.05 vs.AGP)。乙酰甲胆碱(10(-10)-10(-7)mol/kg)或辣椒素(0.01-10.0微克/kg)对NGP和AGP的反应相似。在AGPS中，过度呼吸抑制HIB，直到呼吸暂停后。静脉注射乙酰胆碱(10(-10)-10(-7)mol/kg)，以确定NGPs和AGPs的HIB减少是否由增强抑制所致。在BL型硬脑膜外通气术和暖湿化气体硬脑膜下呼吸暂停期间。AGP和NGP对乙酰胆碱的反应受到类似程度的抑制。我们的结论是，在豚鼠出生后不久，HIB显著减少，并在成熟过程中显著增加。
Adolescent guinea pigs (AGPs) demonstrate dry gas hyperpnea-induced bronchoconstriction (HIB) that shares key features with HIB in humans with asthma. The airways of immature animals exhibit enhanced reactivity to diverse types of stimulation. We tested whether dry gas HIB is also increased in newborn guinea pigs (NGPs). We quantified HIB as the fractional increase of respiratory system resistance (Rrs) over baseline (BL) in five 4- to 7-day-old NGPs after 10 min of hyperpnea, as well as changes in Rrs elicited by intravenous methacholine or capsaicin, and compared these responses with those of AGPs. During hyperpnea, analogous stimuli were delivered by mechanically imposing hyperpnea at 3.0, 4.5, and 6.0 times quiet eucapnic minute ventilation (VE). In AGPs, hyperpnea caused significant bronchoconstriction that increased with VE; peak fractional increase of Rrs was 7.6 +/- 2.0 times BL. In contrast, hyperpnea caused insignificant bronchoconstriction in NGPs (1.4 +/- 0.2 times BL after the largest VE; P < 0.05 vs. AGP). Responses elicited by methacholine (10(-10)-10(-7) mol/kg) or capsaicin (0.01–10.0 microgram/kg) were similar in NGPs and AGPs. In AGPs, hyperpnea suppressed HIB until posthyperpnea. To determine whether the reduced HIB of NGPs was caused by enhanced suppression, NGPs and AGPs were administered acetylcholine (10(-10)-10(-7) mol/kg i.v.) during BL eucapnic ventilation and during eucapnic hyperpnea with warm humidified gas. Responses to acetylcholine were suppressed in AGPs and NGPs to a similar degree. We conclude that HIB is markedly diminished shortly after birth in guinea pigs and that it increases substantially during maturation.(ABSTRACT TRUNCATED AT 250 WORDS)