Direct evidence of electron flow via the heme c group for the direct electron transfer reaction of fructose dehydrogenase using a silver nanoparticle-modified electrode
Direct evidence of electron flow via the heme c group for the direct electron transfer reaction of fructose dehydrogenase using a silver nanoparticle-modified electrode
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DOI：
10.1016/j.elecom.2009.06.012
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发表时间：
2009-08-01
影响因子：
5.4
通讯作者：
Ohno, Hiroyuki
中科院分区：
文献类型：
--
作者：
Murata, Kenichi;Suzuki, Masato;Ohno, Hiroyuki
利用循环伏安法和表面增强共振拉曼散射（SERRS）技术研究了葡萄糖醛酸菌果糖脱氢酶（FDH）在链烷硫醇修饰的银纳米粒子（AgNPs）上的直接电子转移反应。使用循环伏安法，在没有介体的情况下，在D-果糖的存在下，从大约-100 mV（相对于Ag/AgCl，3 M NaCl）的电势观察到催化氧化电流（基于FDH的直接电子转移反应）。溶液中FDH的SERRS光谱和共振拉曼光谱的比较表明，固定后的血红素c位点保留其六配位的低自旋血红素。此外，SERRS还证明吸附的FDH的血红素c是酶内的电子转移位点。(C)2009 Elsevier B. V.保留所有权利。
The direct electron transfer reaction of fructose dehydrogenase (FDH) from Gluconobacter sp. on alkane-thiol-modified silver nanoparticles (AgNPs) was examined using cyclic voltammetry and surface-enhanced resonance Raman scattering (SERRS). Using cyclic voltammetry, catalytic oxidation currents (based on the direct electron transfer reaction of FDH) were observed from a potential of approximately -100 mV (vs. Ag/AgCl, 3 M NaCl) in the presence of D-fructose, without a mediator. A comparison of the SERRS spectra and the resonance Raman spectra of FDH in solution indicated that the heme c site retained its six-coordinated low-spin heme after immobilization. Moreover, SERRS also demonstrated that the heme c of the adsorbed FDH was the electron transfer site within the enzyme. (C) 2009 Elsevier B.V. All rights reserved.