Possible association between amniotic fluid micro-organism infection and microflora in the mouth
Possible association between amniotic fluid micro-organism infection and microflora in the mouth
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DOI：
10.1111/j.1471-0528.2002.01349.x
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发表时间：
2002-05-01
影响因子：
5.8
通讯作者：
Allaker, RP
中科院分区：
文献类型：
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作者：
Bearfield, C;Davenport, ES;Allaker, RP
目的 确定羊膜腔中是否发现口腔细菌。设计基于实验室的临床样本分析。地点为白教堂皇家伦敦医院。人群 48 名参加选择性剖腹产手术的女性。方法 从胎膜完整的女性身上采集牙菌斑、高位阴道拭子、羊水和绒毛羊膜组织。主要结果指标 使用培养和显微镜检查样本中是否存在微生物。通过聚合酶链式反应 (PCR) 分析羊水中是否存在大多数真细菌特有的普遍存在的 16S rRNA 基因。使用针对正常口腔微生物群（链球菌属和具核梭杆菌）一部分的细菌分类群的 PCR 属和种特异性引物对样品进行分析。测量羊水中前列腺素E-2和细胞因子的水平。结果羊水中通用细菌PCR、链球菌属阳性。 PCR 和具核梭菌 PCR 分别占 34/48、20/48 和 7/48 例。链球菌属分别从 48/48、14/48、0/48 和 29/48、6/48、0/48 受试者的牙菌斑、阴道和羊水中培养出具核梭杆菌和具核梭菌。研究发现，微生物 DNA（通用 DNA 和核梭菌 DNA）的检测与既往妊娠的并发症（包括流产、宫内死亡、新生儿死亡、早产和胎膜早破）之间存在显着相关性（分别为 P < 0.05 和 P < 0.01）。前列腺素 E-2 和细胞因子水平（IL-1α 除外）在有感染证据和无感染证据的女性之间没有显着差异。 结论 结果表明链球菌属。羊水中的具核梭杆菌可能源自口腔。
Objective To determine whether oral bacteria are found in the amniotic cavity.Design Laboratory based analysis of clinical samples.Setting Royal London Hospital, Whitechapel.Population Forty-eight women attending for elective caesarean section.Methods Dental plaque, a high vaginal swab, amniotic fluid and chorioamnion tissue were taken from women with intact membranes.Main outcome measures Samples were investigated using culture and microscopy for the presence of microorganisms. Amniotic fluid was analysed by polymerase chain reaction (PCR) for the presence of the ubiquitous 16S rRNA gene specific to most eubacteria. Samples were analysed using PCR genus and species specific primers directed to bacterial taxa found as part of the normal oral microflora (Streptococcus spp. and Fusobacterium nucleatum). Levels of prostaglandin E-2 and cytokines were measured in amniotic fluid.Results Amniotic fluid was positive for universal bacteria PCR, Streptococcus spp. PCR and F. nucleatum PCR in 34/48, 20/48 and 7/48 of cases, respectively. Streptococcus spp. and F. nucleatum were cultured from the dental plaque, vagina and amniotic fluid of 48/48, 14/48, 0/48 and 29/48, 6/48, 0/48 subjects, respectively. A significant association was found between detection of microbial DNA (universal and F. nucletum) and complications in previous pregnancies including miscarriage, intrauterine death, neonatal death, preterm delivery and premature rupture of membranes (P < 0.05 and P < 0.01, respectively). Prostaglandin E-2 and cytokine levels, with the exception of IL-1alpha, were not significantly different between women with and without evidence of infection.Conclusions The results indicate that Streptococcus spp. and F. nucleatum in the amniotic fluid may have an oral origin.