Pontine-ventral respiratory column interactions through raphe circuits detected using multi-array spike train recordings.
Pontine-ventral respiratory column interactions through raphe circuits detected using multi-array spike train recordings.
复制标题
使用多阵列尖峰序列记录检测到通过中缝回路的脑桥腹侧呼吸柱相互作用。
DOI：
10.1152/jn.91305.2008
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发表时间：
2009
影响因子：
2.5
通讯作者：
Lindsey,BruceG
中科院分区：
文献类型：
--
作者：
Nuding,SarahC;Segers,LaurenS;Baekey,DavidM;Dick,ThomasE;Solomon,IreneC;Shannon,Roger;Morris,KendallF;Lindsey,BruceG
最近，西格斯等人。确定了腹外侧呼吸柱（VRC）和脑桥呼吸群（PRG）之间的功能连接。检测到的少突触相互作用的明显稀疏性促使人们考虑这两个区域之间的其他潜在功能通路。我们在这里报告了 PRG 和 VRC 之间通过中间脑干中线中缝神经元“间接”串行功能联系的证据。使用微电极阵列记录来自 11 只去大脑、迷走神经切断、神经肌肉阻断、通气猫的总共 145 个 PRG、282 个 VRC 和 340 个中线神经元的尖峰序列组。对包含至少一个中缝细胞的 13,843 对神经元组成的尖峰序列进行呼吸调节和短时尺度相关性的筛选。在 7.2% 的 raphé-raphé (291/4,021)、4.3% 的 VRC-raphé (292/6,755) 和 4.0% 的 PRG-raphé (124/3,067) 神经元对中检测到显着的相关图特征。表明共同影响的中心峰是中缝神经元对之间相关性中最常见的特征，而相关的中缝-PRG和中缝-VRC神经元对主要显示出偏移的峰和谷，这些特征表明一个神经元对另一个神经元的突触影响很少。总体而言，偏移相关图特征为 33 个 VRC-to-raphé-to-PRG 和 45 个 PRG-to-raphé-to-VRC 相关链接链以及一个或两个中间 raphé 神经元提供了证据。结果支持呼吸网络架构，其中并行的 VRC-to-PRG 和 PRG-to-VRC 链路通过干预中线回路进行操作，并表明中缝神经元有助于 PRG 神经元的呼吸调节，并通过对 PRG 和 VRC 的协调发散动作来塑造呼吸运动模式。
Recently, Segers et al. identified functional connectivity between the ventrolateral respiratory column (VRC) and the pontine respiratory group (PRG). The apparent sparseness of detected paucisynaptic interactions motivated consideration of other potential functional pathways between these two regions. We report here evidence for “indirect” serial functional linkages between the PRG and VRC via intermediary brain stem midline raphé neurons. Arrays of microelectrodes were used to record sets of spike trains from a total of 145 PRG, 282 VRC, and 340 midline neurons in 11 decerebrate, vagotomized, neuromuscularly blocked, ventilated cats. Spike trains of 13,843 pairs of neurons that included at least one raphé cell were screened for respiratory modulation and short-time scale correlations. Significant correlogram features were detected in 7.2% of raphé–raphé (291/4,021), 4.3% of VRC–raphé (292/6,755), and 4.0% of the PRG–raphé (124/3,067) neuron pairs. Central peaks indicative of shared influences were the most common feature in correlations between pairs of raphé neurons, whereas correlated raphé–PRG and raphé–VRC neuron pairs displayed predominantly offset peaks and troughs, features suggesting a paucisynaptic influence of one neuron on the other. Overall, offset correlogram features provided evidence for 33 VRC-to-raphé-to-PRG and 45 PRG-to-raphé-to-VRC correlational linkage chains with one or two intermediate raphé neurons. The results support a respiratory network architecture with parallel VRC-to-PRG and PRG-to-VRC links operating through intervening midline circuits, and suggest that raphé neurons contribute to the respiratory modulation of PRG neurons and shape the respiratory motor pattern through coordinated divergent actions on both the PRG and VRC.