Preparation and characterization of galactosylated alginate-chitosan oligomer microcapsule for hepatocytes microencapsulation.
Preparation and characterization of galactosylated alginate-chitosan oligomer microcapsule for hepatocytes microencapsulation.
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DOI：
10.1016/j.carbpol.2014.06.025
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发表时间：
2014-11
影响因子：
11.2
通讯作者：
Meng Tian;B. Han;Hong Tan;C. You
中科院分区：
文献类型：
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作者：
Meng Tian;B. Han;Hong Tan;C. You
制备了半乳糖基海藻酸盐-壳聚糖低聚物微胶囊，为肝细胞微胶囊化提供了足够的机械稳定性、选择性通透性和合适的三维微环境。微胶囊具有独特的非对称膜结构，致密层位于内表面，向外表面逐渐减少。当GA含量低于50%时，获得稳定的微胶囊，渗透率随GA含量的增加而增加。通过调节膜的孔隙度和厚度，实现了机械稳定性和渗透性之间的平衡。最佳微胶囊具有选择性通透性，可有效阻断免疫球蛋白g，同时有效地运输人血清白蛋白。肝细胞表现出高且长期的活力（>92%）、可增殖性、多细胞球体形态、以及微胶囊中肝脏特异性功能的增强，其中半乳糖部分提供化学线索以支持细胞-基质相互作用，而微胶囊的3D结构表现物理线索以促进细胞-细胞相互作用。
Galactosylated alginate (GA)–chitosan oligomer microcapsule was prepared to provide a sufficient mechanical stability, a selective permeability and an appropriate three-dimensional (3D) microenvironment for hepatocytes microencapsulation. The microcapsule has a unique asymmetric membrane structure, with a dense layer located in the inner surface and gradually decreasing toward the outside surface. The stable microcapsule was obtained when GA lower than 50%, while the permeability was increased with increasing of GA. A balance between mechanical stability and permeability was achieved through modulating membrane porosity and thickness. The optimal microcapsule displays a selective permeability allowing efficient transport of human serum albumin while effectively blocking immunoglobulin G. Hepatocytes exhibited high and long term viability (>92%), proliferability, multicellular spheroid morphology, and enhancement of liver-specific functions in the microcapsule wherein galactose moieties present chemical cues to support cell–matrix interactions while the 3D structure of the microcapsule behaves physical cues to facilitate cell–cell interactions.